REL 2025-01-24 2025-10-23 2025-10-23 0000-0003-3468-3316 Karin Pernet-Gallay Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, F-38000 Grenoble, France Grenoble France 38700 0000-0003-1824-7899 Lucas Benoit Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, F-38000 Grenoble, France Grenoble France 38700 0000-0003-3468-3316 Karin Pernet-Gallay Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, F-38000 Grenoble, France Grenoble France 38700 0000-0003-1069-1602 Andrea Volterra Department of Fundamental Neurosciences, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland Lausanne Switzerland 1005 Benoit LB Pernet-Gallay KPG Jouneau PHJ 258.2 10.6019/EMPIAR-12557 FIB-SEM tissue Benoit LB Hristovska IH Liaudet NL De-Ceglia RDC Jouneau PHJ Fertin AF Litvin DL Telley LT Jevtic JM Bergami MB Usson YU Hisatsune CH Mikoshiba KM Pernet-Gallay KPG Volterra AV English

This archive regroups the different raw FIB/SEM stacks used in the publication. Stacks 1, 2, 3, and IP3Rs immunogold are not aligned and are furnishe as a tif sequence folder, whereas Cx43 stacks have been aligned and is furnished as a mutlipage tif. The stack of 2 images was acquired under conditions with an isotropic voxel size of 4 nm.

Unaligned mutli-frame FIB/SEM micrograph of the mice molecular layer of the hippocampus. /data/ESB_Stack_1 micrographs - multiframe TIFF TIFF 2501 1 1 3301 UNSIGNED BYTE 3266 40.0 2761 40.0

Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication

data/ESB_Stack_1/slice_00001_z=0.0325um.tif Unaligned mutli-frame FIB/SEM micrograph of the mice molecular layer of the hippocampus. /data/ESB_Stack_2 micrographs - multiframe TIFF TIFF 3058 1 1 3058 UNSIGNED 16 BIT INTEGER 8768 40.0 8768 40.0

Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication

data/ESB_Stack_2/slice_00001_z=0.0175um.tif Unaligned mutli-frame FIB/SEM micrograph of the mice molecular layer of the hippocampus. /data/ESB_Stack_3 micrographs - multiframe TIFF TIFF 1108 1 1 1108 UNSIGNED 16 BIT INTEGER 7488 40.0 7488 40.0

Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication

data/ESB_Stack_3/slice_00001_z=0.0196um.tif Unaligned multi-frame FIB/SEM micrograph of the mouse molecular layer of the hippocampus after immunogold pre-embedding for IP3Rs. /data/ESB_IP3Rs_Immunogold micrographs - multiframe TIFF TIFF 3301 1 1 3301 UNSIGNED 16 BIT INTEGER 5504 40.0 5504 40.0

Unaligned FIB-SEM dataset of the mouse dentate gyrus of the hippocampus with immunogold staining acquired at 4 nm isotropic voxel size, deposited as a single-frame image sequence in TIFF format. Details of the imaging procedure and exploitation of the dataset are available in the original publication

data/ESB_IP3Rs_Immunogold/slice_00001_z=0.0607um.tif Aligned multi-frame (as a multipage tif) FIB/SEM micrograph of the mouse molecular layer of the hippocampus after immunogold pre-embedding for Cx43 /data/ESB_Cx43_Immunogold micrographs - multiframe TIFF TIFF 2395 1 1 2395 UNSIGNED 16 BIT INTEGER 3599 40.0 4342 40.0

Aligned and cropped FIB-SEM dataset of the mouse dentate gyrus of the hippocampus with immunogold staining acquired at 4 nm isotropic voxel size, deposited as a multipage TIFF . Details of the imaging procedure and exploitation of the dataset are available in the original publication

data/ESB_Cx43_Immunogold/Stack_750_04_140_2395_TM1b (4 nm).tif