REL
2025-02-10
2025-07-25
2025-07-25
Cryo-electron micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine
0000-0001-9976-5828
Jacquelyn
Renee
Roberts
Department of Biological Chemistry, University of Michigan
1136 Catherine St
Ann Arbor
Michigan
United States
48109
0000-0003-1750-4793
Melanie
D
Ohi
Life Sciences Institute, University of Michigan
210 Washtenaw Ave
Ann Arbor
Michigan
United States
48109
0000-0002-7762-9793
Shoji
Maeda
Department of Pharmacology, University of Michigan School of Medicine
1150 W Medical Center Dr
Ann Arbor
Michigan
United States
48109
Roberts JR
National Institutes of Health (NIH)
S10OD030275
United States
4.0
10.6019/EMPIAR-12549
EMDB
molecule
EMD-42496
Roberts JR
Horibata Y
Kwarcinski FE
Lam V
Raczkowski AM
Hubbard A
White B
Sugimoto H
Tall GG
Ohi MD
Maeda S
Structural basis for catalysis and selectivity of phospholipid synthesis by eukaryotic choline-phosphotransferase
Nature communications
Nat Commun
1
16
2025
English
10.1038/s41467-024-55673-1
39747155
CryoEM datasets of yeast cholinephosphotransferase in complex with CDP choline and phosphatidylcholine. A total of 11,596 multi-frame micrographs (each consisting of 60 frames in TIF format) were acquired. This includes three datasets without CHAPS, comprising 9,074 micrographs, and one dataset with 2,522 micrographs collected in the presence of 3.25 mM CHAPS to mitigate preferred orientation. [11,596 total multi-frame micrographs composed of 60 frames each in TIF format].
Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 1)
/data/20230215_frames
micrographs - multiframe
TIFF
TIFF
3566
60
1
60
UNSIGNED BYTE
5760
1.09
4092
1.09
Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.
data/20230215_frames/*.tif
Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 2)
/data/20230222_frames
micrographs - multiframe
TIFF
TIFF
2563
60
1
60
UNSIGNED BYTE
5760
1.08
4092
1.08
Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.
data/20230222_frames/*.tif
Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 3)
/data/20230301_frames
micrographs - multiframe
TIFF
TIFF
2943
60
1
60
UNSIGNED BYTE
5760
1.08
4092
1.08
Micrographs are unaligned 60 raw movie frames accumulating a total dose of 57 electrons per square angstrom in a 3.47 second exposure.
data/20230301_frames/*.tif
Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine with 3.25 mM CHAPS added before plunging (dataset 4)
/data/20230302_CHAPS_frames
micrographs - multiframe
TIFF
TIFF
2522
60
1
60
UNSIGNED BYTE
5760
1.08
4092
1.08
3.25 mM CHAPS added before plunging to ameliorate preferred orientation. Micrographs are unaligned 60 raw movie frames accumulating a total dose of 61 electrons per square angstrom in a 3.79 second exposure.
data/20230302_CHAPS_frames/*.tif