REL 2025-01-10 2025-03-17 2025-03-17 Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import 0000-0003-2580-4622 Ya-Ting Chang Department of Integrative Structural and Computational Biology, The Scripps Research Institute 10550 N Torrey Pines Rd La Jolla California United States 92037 0000-0003-2580-4622 Ya-Ting Chang Department of Integrative Structural and Computational Biology, The Scripps Research Institute 10550 N Torrey Pines Rd La Jolla California United States 92037 Chang YT Barad BA Rahmani H Zid BM Grotjahn DA 764.2 10.6019/EMPIAR-12534 EMDB cell EMD-48751 EMD-48752 Chang YT Barad BA Rahmani H Zid BM Grotjahn DA Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import bioRxiv United States 2024 English 10.1101/2024.07.17.604013 39071314 Raw unaligned frame and metadata for each tilt in tilt-series acquired on CHX-treated S. cerevisiae cryo-FIB lamellae /data/EMPIAR_upload/Frames micrographs - multiframe TIFF TIFF 462 11 UNSIGNED 16 BIT INTEGER 5760 1.663 4092 1.663
Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding count reference files (mrc file) are in the subfolders of the EMPIAR_upload/Frames folder. The number of frames per image varies from 38 to 42. Subfolder names with YTC041_1, YTC041_2, and YTC042_2 are datasets acquired on S. cerevisiae grown in YPG. Subfolder names with YTC043_1 and YTC043_2 are datasets acquired on S. cerevisiae grown in YPD.
 Unaligned tilt series stack of cryo-electron tomography acquired on CHX-treated S. cerevisiae cryo-FIB lamellae /data/EMPIAR_upload/Tilt_series tilt series MRC MRC 38 11 32 BIT FLOAT 5760 1.663 4092 1.663
The tilt series stacks (.st file) and rawtlt files (in Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the EMPIAR_upload/PACE_mdoc folder. Tilt series stacks are then aligned in IMOD.
 Reconstructed tomograms of CHX-treated S. cerevisiae lamellae used for segmentation of mitochondria /data/EMPIAR_upload/Reconstructed_tomograms reconstructed volumes MRC MRC 38 1 32 BIT FLOAT 682 9.98 960 9.98
Bin6 tomograms are reconstructed from Warp after IMOD alignment. The coordinates of 80S ribosome and co-translating ribosome are provided in the EMPIAR_upload/Particle_lists folder.
data/EMPIAR_upload/Voxel_segmentations Voxel segmentations of mitochondrial outer and inner membrane data/EMPIAR_upload/Reconstructed_tomograms MRC data/EMPIAR_upload/Surfaces Polygon Surface meshes generated from voxel segmentations using 'Surface_Morphometrics' for all membranes from mitochondria data/EMPIAR_upload/Reconstructed_tomograms STL Raw unaligned frame and metadata for each tilt in tilt-series acquired on vehicle-treated S. cerevisiae cryo-FIB lamellae /data/EMPIAR_upload_nonCHX/Frames micrographs - multiframe TIFF TIFF 492 12 UNSIGNED 16 BIT INTEGER 5760 2.638 4092 2.638
Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding count reference files (mrc file) are in the subfolders of the EMPIAR_upload_nonCHX/Frames folder. The number of frames per image varies from 39 to 41. Subfolder name with YTC009_3 is the dataset acquired on S. cerevisiae grown in YPG, and subfolder name with YTC025_2 is the dataset acquired on S. cerevisiae grown in YPD.
 Unaligned tilt series stack of cryo-electron tomography acquired on vehicle-treated S. cerevisiae cryo-FIB lamellae /data/EMPIAR_upload_nonCHX/Tilt_series tilt series MRC MRC 49 12 32 BIT FLOAT 5760 2.638 4092 2.638
The tilt series stacks (.st file) and rawtlt files (in EMPIAR_upload_nonCHX/Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the EMPIAR_upload_nonCHX/mdocs folder. Tilt series stacks are then aligned in IMOD.
 Reconstructed tomograms of vehicle-treated S. cerevisiae lamellae used for segmentation of mitochondria /data/EMPIAR_upload_nonCHX/Reconstructed_tomograms reconstructed volumes MRC MRC 49 1 32 BIT FLOAT 682 15.83 960 15.83
Bin6 tomograms are reconstructed from Warp after IMOD alignment. The coordinates of 80S ribosome and co-translating ribosome are provided in the EMPIAR_upload_nonCHX/Particle_lists folder.
data/EMPIAR_upload_nonCHX/Voxel_segmentations Voxel segmentations of mitochondrial outer and inner membrane data/EMPIAR_upload_nonCHX/Reconstructed_tomograms MRC data/EMPIAR_upload_nonCHX/Surfaces Polygon Surface meshes generated from voxel segmentations using 'Surface_Morphometrics' for all membranes from mitochondria data/EMPIAR_upload_nonCHX/Reconstructed_tomograms STL