<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12499" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-12-19</depositionDate>
            <releaseDate>2025-04-07</releaseDate>
            <updateDate>2025-04-07</updateDate>
        </keyDates>
        <title>Dynamic microtubule plus end architecture in presence of EB3.</title>
        <correspondingAuthor private="true">
            <authorORCID>0009-0000-0040-6770</authorORCID>
            <firstName>Robin</firstName>
            <lastName>Hoogebeen</lastName>
            <organization type="academic">Utrecht University</organization>
            <street>Padualaan 8</street>
            <townOrCity>Utrecht</townOrCity>
            <stateOrProvince>Utrecht</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>3584 CH</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6129-1882</authorORCID>
            <firstName>Stuart</firstName>
            <lastName>Howes</lastName>
            <organization type="academic">Utrecht University</organization>
            <street>Universiteitsweg 99</street>
            <townOrCity>Utrecht</townOrCity>
            <country>Netherlands</country>
            <postOrZipCode>3584 CG</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-9048-8614</authorORCID>
            <firstName>Anna</firstName>
            <lastName>Akhmanova</lastName>
            <organization type="academic">Utrecht University</organization>
            <street>Padualaan 8</street>
            <townOrCity>Utrecht</townOrCity>
            <stateOrProvince>Utrecht</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>3584 CH</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-6953-4607">Saunders H.A.J.</author>
            <author authorORCID="0000-0002-6781-5736">Van den Berg C.M.</author>
            <author authorORCID="0009-0000-0040-6770">Hoogebeen R.A.</author>
            <author>Schweizer D.</author>
            <author authorORCID="0000-0002-8374-689X">Stecker K.E.</author>
            <author authorORCID="0000-0002-5178-8163">Roepman R.</author>
            <author authorORCID="0000-0001-6129-1882">Howes S.C.</author>
            <author authorORCID="0000-0002-9048-8614">Akhmanova A.</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">75.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-12499</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-52359</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0001-6953-4607" order="1">Saunders H.A.J.</author>
                    <author authorORCID="0000-0002-6781-5736" order="2">Van den Berg C.M.</author>
                    <author authorORCID="0009-0000-0040-6770" order="3">Hoogebeen R.A.</author>
                    <author order="4">Schweizer D.</author>
                    <author authorORCID="0000-0002-8374-689X" order="5">Stecker K.E.</author>
                    <author authorORCID="0000-0002-5178-8163" order="6">Roepman R.</author>
                    <author authorORCID="0000-0001-6129-1882" order="7">Howes S.C.</author>
                    <author authorORCID="0000-0002-9048-8614" order="8">Akhmanova A.</author>
                    <title>A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth</title>
                    <journal>Nature Structural &amp; Molecular Biology</journal>
                    <journalAbbreviation>NSMB</journalAbbreviation>
                    <country>Netherlands</country>
                    <language>English</language>
                    <details>The micrographs are non-motion corrected raw movie frames. Models in sff format are tracings of microtubule protofilaments, generated from the subtomograms in the related EMDB entry.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Cryo-ET non-motion corrected multi-frame micrographs of in vitro microtubules grown in presence of EB3</name>
        <directory>/data/control/micrographs</directory>
        <category>tilt series</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>51</numImagesOrTiltSeries>
        <framesPerImage>6</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>2.17</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>2.17</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList>
            <segmentation segmentationId="358">
                <file>data/control/PFtracing_models</file>
                <description>3DMOD tracings of microtubule protofilaments. Tracing was performed on cryoCARE denoised-subtomograms (available in the related EMDB entry) that were generated from the raw tilt series data of this EMPIAR entry.</description>
                <originalFormat>MOD</originalFormat>
            </segmentation>
        </segmentationList>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
