REL 2024-11-11 2026-02-26 2026-02-26 0000-0002-4614-7310 Stefan Lyne Oliver SLAC National Accelerator Laboratory, Division of Cryo-EM and Bioimaging Menlo Park California United States 94025 0000-0002-4614-7310 Stefan Lyne Oliver Stanford University 240 Pasteur Drive Stanford California United States 94305 Oliver SL National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) AI20459 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) AI159375 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM130332 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM150905 United States 3.3 10.6019/EMPIAR-12464 FIB-SEM virus Oliver SL Gain Reference /data/20201229/gainref micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 5760 3.376 4092 3.376

Gain reference for session 20201229.

Unaligned Tilt Series Frames. /data/20201229/frames micrographs - multiframe TIFF TIFF 675 13 UNSIGNED BYTE 5760 3.438 4092 3.438

These are the individual frames for tilt series collected for session 20201229 by cryo-ET of lamella produced by cryo-FIB of VZV infected MeWo cells.

Gain Reference /data/20211103/gainref micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 5760 3.333 4092 3.333

Gain reference for session 20211103.

Unaligned Tilt Series Frames. /data/20211103/frames micrographs - multiframe TIFF TIFF 1619 10 UNSIGNED BYTE 5760 3.44 4092 3.44

These are the individual frames for tilt series collected for session 20211103 by cryo-ET of lamella produced by cryo-FIB of VZV infected MeWo cells.

Gain Reference. /data/20211208/gainref micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 5760 3.333 4092 3.333

Gain reference for session 20211208.

Unaligned Tilt Series Frames. /data/20211208/frames micrographs - multiframe TIFF TIFF 971 7 UNSIGNED BYTE 5760 3.44 4092 3.44

These are the individual frames for tilt series collected for session 20211208 by cryo-ET of lamella produced by cryo-FIB of VZV infected MeWo cells.

Gain Reference. /data/20220507/gainref micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 5760 3.333 4092 3.333

Gain reference for session 20220507.

Unaligned Tilt Series Frames. /data/20220507/frames micrographs - multiframe TIFF TIFF 1373 9 UNSIGNED BYTE 5760 3.44 4092 3.44

These are the individual frames for tilt series collected for session 20220507 by cryo-ET of lamella produced by cryo-FIB of VZV infected MeWo cells.

Gain Reference. /data/20220615/gainref micrographs - single frame MRC MRC 1 1 32 BIT FLOAT 5760 2.646 4092 2.646

Gain reference for session 20220615.

Unaligned Tilt Series Frames. /data/20220615/frames micrographs - multiframe TIFF TIFF 838 6 UNSIGNED BYTE 5760 3.44 4092 3.44

These are the individual frames for tilt series collected for session 20220615 by cryo-ET of lamella produced by cryo-FIB of VZV infected MeWo cells.

EMAN2 Tilt Series /data/EMAN2-tiltseries tilt series MRC MRC 69 60 32 BIT FLOAT 5760 3.44 4092 3.44

Tilt series used to generate cryo-ET maps of the VZV capsid vertex and 3-fold axis of symmetry using EMAN2.

Binned Tomograms /data/relion/tomograms reconstructed volumes MRC MRC 97 200 32 BIT FLOAT 1022 13.76 1440 13.76

These tomograms are binned by 4 and used to generate cryo-ET maps of the VZV portal vertex from B- and C-capsids. Frames per image 200 was used as a place holder as the thickness (Z) for each tomogram varies due to ice thickness of cryo-FIB lamella.

IMOD Aligned Stacks for VZV Capsid Portal Processing /data/relion/imod tilt series MRC MRC 97 60 20 60 16 BIT FLOAT 5760 3.44 4092 3.44

This folder contains a subset of folders from IMOD alignments performed to generate files for input into the Relion 4 tomography pipeline.

Segmentation using Dragonfly /data/Dragonfly reconstructed volumes MRC MRC 1 1 SIGNED 16 BIT INTEGER 1023 13.76 1440 13.76

This folder contains a reconstructed tomogram along with Dragonfly files used for training and segmentation. The individual segments are available in the ChimeraX-Segmentation folder.