REL 2024-11-19 2025-01-29 2025-01-29 0000-0003-3862-8349 Reiya Taniguchi Max Planck Institute of Biophysics Max von Laue Str.3 Frankfurt Germany 60438 0000-0002-7397-1321 Martin Beck Max Planck Institute of Biophysics Max von Laue Str.3 Frankfurt am Main Germany 60438 Taniguchi R Orniacki C Kreysing JP Zila V Zimmerli CE Böhm S Turoňová B Kräusslich H-G Doye V Beck M Max Planck Society none Germany 401.2 10.6019/EMPIAR-12460 EMDB cell EMD-52153 EMD-52154 EMD-52155 Taniguchi R Orniacki C Kreysing JP Zila V Zimmerli CE Böhm S Turoňová B Kräusslich H-G Doye V Beck M Cryo-ET tilt series of FIB-milled wild-type mouse embryonic stem cells /data/mESWT tilt series MRC MRC 161 1 SIGNED 16 BIT INTEGER 5760 2.682 4092 2.682

Images were recorded as movies of 10 frames and were motion-corrected on-the-fly in SerialEM. Tilt series were acquired from the stage pretilt angle of 8 degree, and images were collected using a dose-symmetric acquisition scheme with 2 degree increments, up to +64 or 66 and -52 degrees.