REL 2024-10-07 2025-01-17 2025-01-17 CryoEM of E. coli RNA polymerase and lambda PR promoter DNA 500 ms after mixing (8 mM CHAPSO) 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 Saecker RM Mueller AU Malone B Chen J Budell WC Dandey VP Maruthi K Mendez JH Molina N Eng ET Yen LY Potter CS Carragher B Darst SA 5.9 10.6019/EMPIAR-12339 EMDB molecule EMD-41433 EMD-41439 EMD-41448 EMD-41437 Saecker RM Mueller AU Malone B Chen J Budell WC Dandey VP Maruthi K Mendez JH Molina N Eng ET Yen LY Potter CS Carragher B Darst SA Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy Nature structural & molecular biology Nat Struct Mol Biol 2024 English 10.1038/s41594-024-01349-9 38951624 Unaligned raw movies /data/tifs micrographs - multiframe TIFF TIFF 27923 50 UNSIGNED BYTE 5760 0.844 4092 0.844
FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs accelerating voltage 300 kV; dose rate 41.86 e-/ A^2/s, frame time 0.03 s (50 frames) with total exposure of 1.5 s, total dose of 62.79 e-/A^2; pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range -0.8 to -2.5 um; gain reference is 21dec23a_26102237_13_4092x5760_norm_0.mrc (see references folder). The entry contains all movies (27,923) collected during the Krios session. In the publication, only a subset of movies (14056 tiffs containing *b3g2*) were processed in detail.
data/tifs/21dec23a_b3g2-500ms_*frames.tif