REL
2024-10-04
2025-04-24
2025-04-24
Yeast P complex spliceosome
0000-0003-4738-9503
Max
Wilkinson
Massachusetts Institute of Technology
75 Ames Street
Cambridge
Massachusetts
United States
02142
0000-0003-4738-9503
Max
Wilkinson
Massachusetts Institute of Technology
75 Ames Street
Cambridge
Massachusetts
United States
02142
Wilkinson ME
Hoskins AA
Howard Hughes Medical Institute (HHMI)
Helen Hay Whitney Postdoctoral Fellowship
United States
7.0
10.6019/EMPIAR-12330
EMDB
molecule
EMD-47157
EMD-47158
EMD-47159
EMD-47160
EMD-47161
EMD-47162
EMD-47163
EMD-47164
Senn KA
Lipinski KA
Zeps NJ
Griffin AF
Wilkinson ME
Hoskins AA
Control of 3’ splice site selection by the yeast splicing factor Fyv6
bioRxiv
Mol Cell
United States
7
2024
10.1101/2024.05.04.592262
38746449
Unaligned multi-frame movies of the yeast P-complex spliceosome, 81000x magnification
/data/movies_grid1
micrographs - multiframe
TIFF
TIFF
15690
40
UNSIGNED BYTE
5760
0.934
4092
0.934
Data for grid 1, collected at the MRC LMB Krios1
Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 81000
Pixel size 0.934 A/pix
Dose 40 e/A2
Exposure rate 25 e/pix/s
Exposure time 1.44s
Fractions 40 by mistake, some of the movies have 41 frames instead. Depending on your processing strategy, these might have to be removed or processed seperately to avoid bugs.
The gain reference "gainref.tiff" is provided. It can be used without rotation or flipping for RELION motion correction.
Metadata are also included for all maps deposited to the EMDB from this dataset. These are direct copies of the "run_data.star" from the Refine3D directory of the RELION project.
data/movies_grid1/movies/GridSquare_*/Data/FoilHole_*_fractions.tiff
data/stateI_emd47157.star
Unaligned multi-frame movies of the yeast P-complex spliceosome, 130000x magnification
/data/movies_grid2
micrographs - multiframe
TIFF
TIFF
35423
40
UNSIGNED BYTE
5760
0.664
4092
0.664
Data for grid 2, collected at the MRC LMB Krios3
Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 130000
Pixel size 0.664 A/pix
Dose 40 e/A2
Exposure rate 25 e/pix/s
Exposure time 0.72s
Fractions 40
The gain reference "gainref.tiff" is provided. It can be used without rotation or flipping for RELION motion correction.
Metadata are also included for all maps deposited to the EMDB from this dataset. These are direct copies of the "run_data.star" from the Refine3D directory of the RELION project.
data/movies_grid2/movies/GridSquare_*/Data/FoilHole_*_fractions.tiff
data/stateI_emd47157.star