REL 2024-10-03 2025-12-08 2025-12-08 0000-0001-9635-3844 Albert Weixlbaumer Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258 1, rue Laurent Fries Illkirch France 67404 0000-0001-7793-4029 Valerie Lamour Department of integrative Structural Biology, IGBMC Illkirch France 67404 0000-0001-9635-3844 Albert Weixlbaumer Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258 1, rue Laurent Fries Illkirch France 67404 Vidmar VV Lamour VL Weixlbaumer AW Agence Nationale de la Recherche (ANR) ANR-21-CE11-0040-01 France Agence Nationale de la Recherche (ANR) ANR-10-INBS-05 France 10.2 10.6019/EMPIAR-12329 EMDB molecule EMD-51259 EMD-51252 EMD-51253 EMD-51254 EMD-51255 EMD-51256 EMD-51257 EMD-51258 EMD-51267 EMD-51265 EMD-51266 Vidmar VV Borde CB Bruno LB Takacs MT Batisse CB Saint-Andre CSA Zhu CZ Espeli OE Lamour VL Weixlbaumer AW

The micrographs are raw movies (50 frames) with gain correction applied; the total electron dose is 56.6 e/A2.

unaligned multi-frame movies of RNAP-TopoI on duplex scaffold /data/movies micrographs - multiframe TIFF TIFF 21561 50 UNSIGNED BYTE 5760 0.839 4092 0.839

300 kV, Cs 2.7 mm, defocus range -0.8 – -2.5 um, total dose 56.60 e/A2 Movie frames are gain-corrected.

data/movies/movies_*/FoilHole_* data/particles/ all good particles of RNAP-TopoI on duplex scaffold /data/particles picked particles - single frame - processed MRC MRC 16044 1 32 BIT FLOAT 360 0.839 360 0.839

Particles were initially picked in crYOLO (Wagner et al., Commun. Biol. 2019) and re-extracted after 2D classification & heterogeneous refinement in cryoSPARC (Punjani et al., Nat. Methods 2017)

data/movies/movies_*/FoilHole_* data/particles/