REL
2024-10-03
2025-11-18
2025-11-18
RNAP-TopoI complex on short-overhang scaffold
0000-0001-9635-3844
Albert
Weixlbaumer
Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258
1, rue Laurent Fries
Illkirch
France
67404
0000-0001-7793-4029
Valerie
Lamour
Department of integrative Structural Biology, IGBMC
Illkirch
France
67404
0000-0001-9635-3844
Albert
Weixlbaumer
Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology - IGBMC - UMR 7104 - U 1258
1, rue Laurent Fries
Illkirch
France
67404
Vidmar VV
Lamour VL
Weixlbaumer AW
Agence Nationale de la Recherche (ANR)
ANR-21-CE11-0040-01
France
Agence Nationale de la Recherche (ANR)
ANR-10-INBS-05
France
1.5
10.6019/EMPIAR-12328
EMDB
molecule
EMD-51270
EMD-51271
Vidmar VV
Borde CB
Bruno LB
Takacs MT
Batisse CB
Saint-Andre CSA
Zhu CZ
Espeli OE
Lamour VL
Weixlbaumer AW
DNA topoisomerase I acts as supercoiling sensor for transcription elongation in E. coli
The micrographs are raw movies (39 frames) without gain correction (gain.mrc is provided); total electron dose is 55.55 e/A2.
unaligned multi-frame movies of RNAP-TopoI on short-overhang scaffold
/data/movies
micrographs - multiframe
TIFF
TIFF
7254
39
UNSIGNED BYTE
5760
0.862
4092
0.862
300 kV, Cs 0.01 mm, defocus range -1.0 – -2.0 um, total dose 55.55 e/A2
Gain reference file is provided in data/movies/gain.mrc
data/movies/movies-*/EC13-6*
data/particles/
picked particles of RNAP-TopoI on short-overhang scaffold
/data/particles
picked particles - single frame - unprocessed
MRCS
MRCS
7250
1
32 BIT FLOAT
360
0.862
360
0.862
Particles were picked and extracted in WARP (Tegunov & Cramer, Nat. Methods 2019) as a part of on-the-fly processing pipeline at the IGBMC, Strasbourg, France.
data/movies/movies-*/EC13-6*
data/particles/