REL
2024-10-01
2025-06-02
2025-06-02
Cryo-EM micrographs for the DRT2 reverse transcriptase / ncRNA complex in resting and elongating states
0000-0003-4738-9503
Max
Wilkinson
Broad Institute of MIT and Harvard
75 Ames St
Cambridge
Massachusetts
United States
02142
0000-0003-0178-7995
Feng
Zhang
Broad Institute of MIT and Harvard
Cambridge
United States
02142
Wilkinson ME
Li D
Gao A
Macrae RK
Zhang F
Howard Hughes Medical Institute (HHMI)
NA
United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)
R01HG009761
United States
6.2
10.6019/EMPIAR-12322
EMDB
molecule
EMD-45085
EMD-45086
Wilkinson ME
Li D
Gao A
Macrae RK
Zhang F
Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA
Science (New York, N.Y.)
Science
2024
English
10.1126/science.adq3977
39208082
Unaligned multi-frame movies of DRT2 ribonucleoprotein complex in the resting state (no added dNTPs)
/data/movies_resting
micrographs - multiframe
TIFF
TIFF
28211
41
1
41
UNSIGNED BYTE
5760
0.663
4092
0.663
Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM
Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 130000
Pixel size 0.663 A/pix
Dose 40 e/A2
Exposure rate 19.9 e/pix/s
Exposure time 0.9 s
Fractions 40
Total mics 14220 (grid 1)
13991 (grid 2)
Each grid has its own "gainref.mrc" gain reference file. These weres binned using 'rebin by two' in Digital Micrograph. They need to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can be used as is for cryoSPARC.
particles_resting.star contains metadata for the 230,001 particles refined for the resting state (EMDB 45085).
data/movies_resting/grid*/movies_grid*/GridSquare_*/Data/FoilHole_*_fractions.tiff
data/particles_resting.star
Unaligned multi-frame movies of DRT2 ribonucleoprotein complex in the elongating state (+dNTPs)
/data/movies_elongating
micrographs - multiframe
TIFF
TIFF
18453
41
1
41
UNSIGNED BYTE
5760
0.663
4092
0.663
Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM
Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 130000
Pixel size 0.663 A/pix
Dose 40 e/A2
Exposure rate 20.4
Exposure time 0.87 s
Fractions 40
Total mics 18453
Each grid has its own "gainref.mrc" gain reference file. These were binned using 'rebin by two' in Digital Micrograph. They need to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can be used as is for cryoSPARC.
particles_elongating.star contains metadata for the 136,601 particles refined for the elongating state (EMDB 45086).
data/movies_elongating/movies/GridSquare_*/Data/FoilHole_*_fractions.tiff
data/particles_elongating.star