REL 2024-09-05 2025-06-02 2025-06-02 0000-0001-7780-5278 Christopher P Jones Laboratory of Nucleic Acids, NHLBI, NIH 50 South Drive, MSC 8012; Building 50, Room 2144 Bethesda Maryland United States 20892 0000-0003-4549-1619 Adrian R Ferre-D'Amare Laboratory of Nucleic Acids, NHLBI, NIH 50 South Drive, MSC 8012; Building 50, Room 2144 Bethesda Maryland United States 20892 Jones CP Ferre-D'Amare AR National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) none United States 4.4 10.6019/EMPIAR-12272 SBF-SEM molecule Jones CP Ferré-D'Amaré AR Proceedings of the National Academy of Sciences of the United States of America Proc Natl Acad Sci U S A 14 122 2025 English 10.1073/pnas.2418418122 40172966 Polished particles used for final reconstruction of Rous sarcoma virus straight dimer /data/job1409 picked particles - multiframe - processed MRCS MRC 88523 50 1 50 32 BIT FLOAT 320 0.83 320 0.83

Polished particles used for final map reconstruction for Rous sarcoma virus frameshifting pseudoknot straight dimer. These ~85,000 particles were imported into cryoSPARC for homo/NU refinement jobs. This is the RELION job folder.

data/job1409/shiny.star data/job1409/20220725 Polished particles used for final reconstruction of Rous sarcoma virus bent dimer /data/job1468 picked particles - multiframe - processed MRCS MRC 107620 50 1 50 32 BIT FLOAT 320 0.83 320 0.83

Polished particles used for final map reconstruction for Rous sarcoma virus frameshifting pseudoknot bent dimer. These ~108,000 particles were imported into cryoSPARC for homo/NU refinement jobs. This is the RELION job folder.

data/job1468/shiny.star data/job1468/20220725 Uncurated micrographs for entire dataset /data/Micrographs micrographs - multiframe TIFF TIFF 10204 50 1 50 UNSIGNED BYTE 11520 0.415 8184 0.415

Uncurated micrographs for the Rous sarcoma virus frameshifting pseudoknot data used to produce particle sets above. Collected at 105,000 magnification, 54.4 e/A2 dose, 50 frames, -0.8-2.0 defocus range on a Titan Krios G1 with Gatan BioQuantum K3 camera and imaging filter (20 eV slit) over 3 days. After motion correction (with 2-fold binning) and CTF estimation in RELION, curation of micrographs produced ~7800 micrographs, from which ~3.4M initial particles were picked with Topaz. Of these, ~400,000 were dimeric particles, which were further classified into "straight" and "bent" particle sets in cryoSPARC. Those were further separated, CTF refined and polished in RELION. These produced the ~88500 straight and ~108000 bent particles also deposited here.

data/Micrographs/*.tif