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        <keyDates>
            <depositionDate>2024-08-29</depositionDate>
            <releaseDate>2025-02-03</releaseDate>
            <updateDate>2025-07-06</updateDate>
        </keyDates>
        <title>E.coli DNA gyrase in complex with cleaved 217 bp substrate DNA (chirally wrapped state) and moxifloxacin</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9325-3207</authorORCID>
            <firstName>Dmitry</firstName>
            <lastName>Ghilarov</lastName>
            <organization type="academic">John Innes Centre</organization>
            <street>Colney Lane</street>
            <townOrCity>Norwich</townOrCity>
            <stateOrProvince>Norfolk</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>NR4 7UH</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9325-3207</authorORCID>
            <firstName>Dmitry</firstName>
            <lastName>Ghilarov</lastName>
            <organization type="academic">John Innes Centre</organization>
            <townOrCity>Norwich</townOrCity>
            <country>United Kingdom</country>
            <postOrZipCode>NR4 7UH</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Michalczyk E.</author>
            <author>Pakosz-Stepien Z</author>
            <author>Liston J.</author>
            <author>Gittins O.</author>
            <author>Liston J.D.</author>
            <author>Pabis M.</author>
            <author>Heddle J.G.</author>
            <author>Ghilarov D.</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Wellcome Trust</fundingBody>
                <code>221868/Z/20/Z</code>
                <country>United Kingdom</country>
            </grantReference>
            <grantReference>
                <fundingBody>Polish National Science Centre</fundingBody>
                <code>OPUS20: 2020/39/B/NZ1/02898</code>
                <country>Poland</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-12255</entryDOI>
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        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-51339</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author order="1">Michalczyk E.</author>
                    <author order="2">Pakosz-Stepien Z</author>
                    <author order="3">Liston J.</author>
                    <author order="4">Gittins O.</author>
                    <author order="5">Liston J.D.</author>
                    <author order="6">Pabis M.</author>
                    <author order="7">Heddle J.G.</author>
                    <author order="8">Ghilarov D.</author>
                    <title>Structure of Escherichia coli DNA gyrase with chirally wrapped DNA supports ratchet-and-pawl mechanism for an ATP-powered supercoiling motor</title>
                    <journal>Proceedings of the Academy of Sciences USA</journal>
                    <journalAbbreviation>PNAS</journalAbbreviation>
                    <country>United States</country>
                    <year>2024</year>
                    <language>English</language>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of E.coli gyrase-Mu217-MFX ternary complex in a chirally wrapped state</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>8508</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.86</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.86</pixelHeight>
        </dimensions>
        <details>Gain-corrected K3 movies compressed in TIFF format, 40 frames each, total dose 40.68 e/A. Further data collection details available in the parameters text file within the same folder.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
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