REL 2024-07-26 2025-02-03 2025-02-03 Cryo-EM images of B. megaterium gas vesicles in thin vitreous monolayers 0000-0003-3721-5104 Stefan T Huber Department of Bionanoscience / TU Delft Van der Maasweg 9 Delft Zuid-Holland Netherlands 2629HZ 0000-0003-3721-5104 Arjen J Jakobi Department of Bionanoscience / TU Delft Van der Maasweg 9 Delft Zuid-Holland Netherlands 2629HZ Huber ST Jakobi AJ 2.3 10.6019/EMPIAR-12228 EMDB molecule EMD-14238 Huber ST Terwiel D Evers WH Maresca D Jakobi AJ Cryo-EM structure of gas vesicles for buoyancy-controlled motility Cell Cell 5 186 975 986.e13 2023 English 10.1016/j.cell.2023.01.041 36868215
4351 super-resolution movies, 1.37A pixel size (later calibrated to ~1.34 using a newer dataset and comparison of helical parameters), 30 e/A2, 60 frames, defocus range from −0.25 to −1.25 μm. Star-file of manual picks of thin 34-42 nm tubes in RELION included.
 Unaligned multi-frame movies of B.megaterium gas vesicles /data/movies micrographs - multiframe TIFF TIFF 4352 60 SIGNED BYTE 11520 0.685 8184 0.685
Unaligned multi-frame movies of B.megaterium gas vesicles (concentation: OD500=3.1) in superresolution mode from a K3 detector. Pixelsize is 1.37A, superres pixelsize is 0.685A. This pixel size was used for processing and in the publication. Subsequent work established that the pixelsize was not perfectly calibrated and should be closer to 1.341A.
data/movies/FoilHole_*_fractions*tiff data/manualpick_starfiles/*star data/manualpick_starfiles