<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12216" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-08-07</depositionDate>
            <releaseDate>2025-05-09</releaseDate>
            <updateDate>2025-05-09</updateDate>
        </keyDates>
        <title>Unaligned multi-frame micrographs of DNA polymerase theta with 3′-overhang DNA with 15-nt polyT</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-2274-118X</authorORCID>
            <firstName>Fumiaki</firstName>
            <lastName>Ito</lastName>
            <organization type="academic">Molecular and Computational Biology, Departments of Biological Sciences, University of Southern California</organization>
            <townOrCity>Los Angeles</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>90089</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <firstName>Xiaojiang</firstName>
            <lastName>Chen</lastName>
            <organization type="academic">Molecular and Computational Biology, Departments of Biological Sciences, University of Southern California</organization>
            <townOrCity>Los Angeles</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>90089</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-2274-118X">Ito F</author>
            <author>Li Z</author>
            <author>Khant HA</author>
            <author>Chen XS</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">2.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-12216</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-43706</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-2274-118X" order="1">Ito F</author>
                    <author order="2">Li Z</author>
                    <author order="3">Minakhin L</author>
                    <author order="4">Khant HA</author>
                    <author order="5">Pomerantz RT</author>
                    <author order="6">Chen XS</author>
                    <title>Structural basis for Polθ-helicase DNA binding and microhomology-mediated end-joining</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>16</volume>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-025-58441-x</externalReferences>
                    <externalReferences type="pubmed">40253368</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of DNA polymerase theta with 3′-overhang DNA with 15-nt polyT</name>
        <directory>/data/Images-Disc1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>JPEG</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7228</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4092</imageWidth>
            <pixelWidth>0.86</pixelWidth>
            <imageHeight>5760</imageHeight>
            <pixelHeight>0.86</pixelHeight>
        </dimensions>
        <details>The gain reference image in mrc format is in the root directory, it needs to be flipped in Y.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Images-Disc1/GridSquare_*/Data/FoilHole_*.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
