REL
2024-04-09
2025-10-15
2025-10-15
Cryo-EM structure of antibody TJ5-13 bound to H3 COBRA NG2 hemagglutinin
0000-0002-2447-146X
John
Vincent
Dzimianski
Department of Biomolecular Engineering, University of California
1156 High Street
Santa Cruz
California
United States
95064
0000-0003-4185-5673
Rebecca
M
DuBois
Department of Biomolecular Engineering, University of California
1156 High Street
Santa Cruz
California
United States
95064
Dzimianski JV
Cruz JM
Balasco Serrao VH
DuBois RM
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
75N93019C00052
United States
29.6
10.6019/EMPIAR-12186
EMDB
molecule
EMD-44305
Dzimianski JV
Nagashima KA
Cruz JM
Sautto GA
O'Rourke SM
Serrão VHB
Ross TM
Mousa JJ
DuBois RM
Assessing the structural boundaries of broadly reactive antibody interactions with diverse H3 influenza hemagglutinin proteins
Journal of virology
J Virol
United States
2025
English
10.1128/jvi.00453-25
40810533
Titan Krios G2 cryo-TEM coupled with a Gatan K3 direct electron detector at an accelerating voltage of 300 kV located at the W.M. Keck Foundation Advanced Microscopy Laboratory - UCSF. The data was collected in SerialEM in counting mode at a nominal magnification of 105,500, pixel size of 0.835 Å/pixel, exposure time of 2 s, and a total dose of 45.8 electrons/Å2. The data from each were combined for a total of 13,223 movies and processed together using CryoSPARC v3.2.
Unaligned multiframes HA-bound to antibodies
/data
picked particles - multiframe - processed
TIFF
TIFF
13223
50
16 BIT FLOAT
5760
0.835
4092
0.835
Micrographs from two data collections collected with the same optics on a Titan Krios G2 cryo-TEM coupled with a Gatan K3 direct electron detector at an accelerating voltage of 300 kV and processed together. The micrographs are the average, without realignment, of raw movie frames collected at a pixel size of 0.835 Angstrom/pixel at an accumulated dose of 45.8 electrons per square Angstrom over a 2 s exposure.