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        <keyDates>
            <depositionDate>2024-06-18</depositionDate>
            <releaseDate>2025-10-10</releaseDate>
            <updateDate>2025-10-10</updateDate>
        </keyDates>
        <title>Single particle cryoelectron microscopy of Human astrovirus 1 spike in complex with neutralizing Fabs 3B4 and 3H4</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-9398-2941</authorORCID>
            <firstName>Vitor Hugo</firstName>
            <lastName>Serrão</lastName>
            <organization type="academic">UCSC Biomolecular cryoEM facility</organization>
            <street>1156 High St</street>
            <townOrCity>Santa Cruz</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>95060</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-4185-5673</authorORCID>
            <firstName>Rebecca</firstName>
            <middleName>M</middleName>
            <lastName>DuBois</lastName>
            <organization type="academic">Biomolecular Engineering Department</organization>
            <street>1156 High St</street>
            <townOrCity>Santa Cruz</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>95060</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-6206-2843">Lanning S</author>
            <author authorORCID="0000-0002-9398-2941">Balasco Serrao VH</author>
            <author authorORCID="0000-0003-4185-5673">DuBois RM</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</fundingBody>
                <code>R01 AI144090</code>
                <country>United States</country>
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        </grantSupport>
        <datasetSize units="TB">26.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-12182</entryDOI>
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        <scale>molecule</scale>
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            <emdbEntry>EMD-45427</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
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                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-6206-2843" order="1">Lanning S</author>
                    <author order="2">Aguilar-Hernández N</author>
                    <author authorORCID="0000-0002-9398-2941" order="3">Serrão VHB</author>
                    <author order="4">López T</author>
                    <author order="5">O'Rourke SM</author>
                    <author authorORCID="0009-0004-2628-3712" order="6">Lentz A</author>
                    <author authorORCID="0000-0001-5543-6117" order="7">Ricemeyer L</author>
                    <author order="8">Espinosa R</author>
                    <author authorORCID="0000-0001-6336-9209" order="9">López S</author>
                    <author authorORCID="0000-0003-3130-4501" order="10">Arias CF</author>
                    <author authorORCID="0000-0003-4185-5673" order="11">DuBois RM</author>
                    <title>Discovery of three novel neutralizing antibody epitopes on the human astrovirus capsid spike and mechanistic insights into virus neutralization</title>
                    <journal>Journal of virology</journal>
                    <journalAbbreviation>J Virol</journalAbbreviation>
                    <country>United States</country>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1128/jvi.01619-24</externalReferences>
                    <externalReferences type="pubmed">39846739</externalReferences>
                    <details>Raw data of Human astrovirus 1 spike in complex with neutralizing Fabs 3B4 and 3H4 collected at PNCC (160263) at 300 kV Krios (CS 2.7 nm) pixel size 0.415 A/pix and total dose of 50 e/A2. Processed data using cryoSPARC workflow.</details>
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        <dataFormat>TIFF</dataFormat>
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        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
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            <pixelWidth>variable</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>variable</pixelHeight>
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        <details>Raw and processed data collected at Krios G3i, 300 kV, 32.26 e/A2 using Gatan K3 Biocontinuum.</details>
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