REL 2024-06-03 2025-05-22 2025-05-22 0000-0002-9479-3800 Mahrukh Usmani Department of Cell Biology, New York University School of Medicine New York NY United States 10016 0000-0003-0624-6178 Gira Bhabha Department of Biology, Johns Hopkins University Baltimore MD United States 21218 0000-0002-2570-0404 Damian C Ekiert Department of Biology, Johns Hopkins University Baltimore MD United States 21218 Usmani M Coudray N Raghu R Ramchandani H Bobe D Kopylov M Zhong ED Ekiert DC Bhabha G American Heart Association 915749 United States Searle Scholars Program SSP-2018-2737 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI147131 United States The Pew Charitable Trusts PEW-00033055 United States NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program U24 GM129539 United States Simons Foundation SF349247 United States NY State Assembly N/A United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 9 P41 GM103310 United States Irma T. Hirschl Career Scientist Award N/A United States 1.1 10.6019/EMPIAR-12177 EMDB cell EMD-45671 EMD-45672 Usmani M Coudray N Riggi M Raghu R Ramchandani H Bobe D Kopylov M Zhong ED Iwasa JH Ekiert DC Bhabha G 10.1101/2024.07.13.603322v1 Raw frames from dataset 3 of microsporidian Encephalitozoon intestinalis /data/Data_Set_3 tilt series TIFF TIFF 94 10 UNSIGNED BYTE 5760 3.431 4092 3.431

Each tilt series is contained within its unique directory with an identifiable name. The folder called 'raw_frames' contains the raw frames and corresponding mdoc field from each tilt series. The aligned tilt series file can be found in 'aligned_tilt_series'.

Unaligned tilt stacks from dataset 3 of microsporidian Encephalitozoon intestinalis /data/Data_Set_3 tilt series MRC MRC 94 1 32 BIT FLOAT 5760 3.431 4092 3.431

Each tilt series is contained within its unique directory with an identifiable name. The folder called 'tilt_series' contains the unaligned tilt stacks with corresponding tilt files. The aligned tilt series can be found in 'aligned_tilt_series'.

Reconstructed tomograms from dataset 3 of microsporidian Encephalitozoon intestinalis /data/Data_Set_3 reconstructed volumes MRC MRC 94 1 32 BIT FLOAT variable variable variable variable

Each tomogram reconstruction is contained within its unique directory with an identifiable name. The folder called 'tomo_rec_4bin_SART' contains the 4 times binned volume reconstructed using aretomo as examples.