REL 2024-02-14 2024-09-26 2025-04-11 SBF SEM images of a notoroid in transverse orientation (Fig S24) 0000-0002-5703-2922 Marie-Charlotte Domart The Francis Crick Institute, Electron Microscopy STP 1 Midland Road London United Kingdom NW1 1AT 0000-0002-5703-2922 Marie-Charlotte Domart The Francis Crick Institute, Electron Microscopy STP Midland road London United Kingdom NW1 1AT Marie-Charlotte Domart MCD Lucy M Collinson LMC 11.5 10.6019/EMPIAR-12161 SBF-SEM tissue Tiago Rito TR Ashley R.G. Libby ARGL Madeleine Demuth MD Marie-Charlotte Domart MCD Jake Cornwall-Scoones JCS James Briscoe JB Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation 10.1038/s41586-024-08332-w
The data in this entry are the ones shown in Fig 6m and Fig S24 of the paper
 Low magnification raw SBF SEM overview images (ROI00) of notoroid in transverse orientation /data/FigS24/ROI00 micrographs - single frame DM4 DM4 50 1 UNSIGNED 16 BIT INTEGER 7000 610.0 7000 610.0
Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 and ROI02 below. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.
 High magnification raw SBF SEM images (ROI01) of notochord in notoroid in transverse orientation /data/FigS24/ROI01 micrographs - single frame DM4 DM4 50 1 UNSIGNED 16 BIT INTEGER 7000 88.0 7000 88.0
High magnification images (ROI01) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.
 High magnification raw SBF SEM images (ROI02) of notochord in notoroid in transverse orientation /data/FigS24/ROI02 micrographs - single frame DM4 DM4 50 1 UNSIGNED 16 BIT INTEGER 7000 83.0 7000 83.0
High magnification images (ROI02) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.
 Survey image showing the relative position of each of the ROIs above /data/Survey-image micrographs - single frame TIFF TIFF 1 1 UNSIGNED BYTE variable variable variable variable
Survey image used to setup the multi ROI run in Digital Micrograph. Shows the position of each ROI.