REL
2024-05-28
2025-01-23
2025-01-23
Cryogenic electron microscopy of blot-plunged acetyl-CoA decarbonylase/synthase
0000-0001-5486-2755
Catherine
L
Drennan
Departments of Chemistry and Biology, Massachusetts Institute of Technology
31 Ames St
Cambridge
MA
United States
02142
0000-0001-5486-2755
Catherine
L
Drennan
Departments of Chemistry and Biology, Massachusetts Institute of Technology
31 Ames St
Cambridge
MA
United States
02142
Biester A
Drennan CL
Howard Hughes Medical Institute (HHMI)
N/A
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM126982
United States
1.8
10.6019/EMPIAR-12108
EMDB
molecule
EMD-45089
Biester A
Grahame DA
Drennan CL
Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy
Proceedings of the National Academy of Sciences of the United States of America
Proc Natl Acad Sci U S A
41
121
2024
English
10.1073/pnas.2410995121
39361653
Unaligned multi-frame micrographs of acetyl-CoA decarbonylase/synthase (ACDS) complex and subcomplexes
/data
micrographs - multiframe
TIFF
TIFF
14078
21
0
20
UNSIGNED BYTE
5760
0.832
4092
0.832
Gain reference:
data/GainsBackup2021-12-10/K3-18030019 Gain Ref.x1.m1.bin2.mrc
Defocus range: -0.8 to -2.1 µM
Total exposure on specimen: 54.53 el/A2
Exposure time: 1.69 s
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3