<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12077" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-04-11</depositionDate>
            <releaseDate>2024-12-02</releaseDate>
            <updateDate>2024-12-02</updateDate>
        </keyDates>
        <title>Single particle dataset of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-8667-6797</authorORCID>
            <firstName>Judy</firstName>
            <lastName>Hirst</lastName>
            <organization type="academic">MRC Mitochondrial Biology Unit, University of Cambridge</organization>
            <street>Keith Peters Building, Cambridge Biomedical Campus</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 0XY</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-8667-6797</authorORCID>
            <firstName>Judy</firstName>
            <lastName>Hirst</lastName>
            <organization type="academic">MRC Mitochondrial Biology Unit, University of Cambridge</organization>
            <street>Keith Peters Building,, Cambridge Biomedical Campus</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 0XY</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-1039-3894">Ivanov BS</author>
            <author authorORCID="0000-0001-6890-6050">Bridges HR</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Medical Research Council (MRC, United Kingdom)</fundingBody>
                <code>MC_UU_00028/1</code>
                <country>United Kingdom</country>
            </grantReference>
            <grantReference>
                <fundingBody>Medical Research Council (MRC, United Kingdom)</fundingBody>
                <code>MC_UU_00015/2</code>
                <country>United Kingdom</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">6.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-12077</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-18324</emdbEntry>
            <emdbEntry>EMD-18325</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0002-1039-3894" order="1">Ivanov BS</author>
                    <author authorORCID="0000-0001-6890-6050" order="2">Bridges HR</author>
                    <author authorORCID="0000-0001-8667-6797" order="3">Hirst J</author>
                    <author order="4">Jarman OD</author>
                    <title>Structure of the turnover-ready state of an ancestral respiratory complex I</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United Kingdom</country>
                    <externalReferences type="doi">10.1038/s41467-024-53679-3</externalReferences>
                    <externalReferences type="pubmed">39472559</externalReferences>
                    <details>The micrographs are non-gain corrected, averaged of 40 unaligned frames, with a dose rate of 19.43 e-/Å^2/s over 2.4 s, giving a total dose of 46.6 e-/Å^2.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>16814</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.533</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.533</pixelHeight>
        </dimensions>
        <details>Non-gain corrected micrographs were imaged using a Gatan K3 in super-resolution mode and with a GIF BioQuantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 2.4 s exposures captured in 40 frames and a total dose of 46.6 electrons Å-2. Dataset also contains polished (0.533 pixel size) and re-polished (0.534 pixel size) particle stacks and gain reference files.</details>
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        <micrographsFilePattern>data/sym_link_EMPIAR_Grid1-2</micrographsFilePattern>
        <pickedParticlesFilePattern>data/sym_link_EMPIAR_Grid1-2</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/sym_link_EMPIAR_Grid1-2</pickedParticlesDirectory>
    </imageSet>
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