REL
2024-05-03
2024-05-16
2024-05-16
The cryo-electron microscopy structure of a Vitiosangium bacterial gasdermin in an slinky-like oligomeric conformation
0000-0002-4040-9797
Alex
Geoffrey
Johnson
Harvard Medical School, Dana-Farber Cancer Institute
1 Jimmy Fund Way
Boston
Massachusetts
United States
02446
0000-0002-4943-733X
Philip
J
Kranzusch
Harvard Medical School, Dana-Farber Cancer Institute
1 Jimmy Fund Way
Boston
Massachusetts
United States
02115
0000-0001-7768-746X
Gerhard
Hummer
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
n/a
Germany
60439
Johnson AG
Mayer ML
Schaefer SL
McNamara-Bordewick NK
Hummer G
Kranzusch PJ
National Institutes of Health (NIH)
1DP2GM146250-01
United States
2.1
10.6019/EMPIAR-12073
EMDB
molecule
EMD-40570
Johnson AG
Mayer ML
Schaefer SL
McNamara-Bordewick NK
Hummer G
Kranzusch PJ
Structure and assembly of a bacterial gasdermin pore
Nature
Nature
United Kingdom
8008
628
657
663
2024
English
10.1038/s41586-024-07216-3
38509367
8,156 movies were acquired at a pixel size of 1.06 Å, a total dose of 51.8 e− Å−2, dose per frame of 1.04 e− Å−2 at a defocus range of −0.7 to −2.0 μm. The data was collected on a Krios microscope with the spherical aberration was 2.7 and accelerating voltage 300. Pixel size is 1.06.
Raw micrographs of Vitiosangium bacterial gasdermin slinky-like oligomers
/data/Raw_data
micrographs - multiframe
TIFF
TIFF
8156
50
UNSIGNED BYTE
4092
1.06
5760
1.06