Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA

REL 2024-03-20 2024-06-12 2024-06-12 Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA 0000-0003-1144-9877 Ben Luisi Department of Biochemistry, Sanger Building, University of Cambridge 80 Tennis Court Road Cambridge Cambridgeshire United Kingdom CB2 1GA 0000-0003-1144-9877 Ben Luisi Department of Biochemistry, Sanger Building, University of Cambridge 80 Tennis Court Road Cambridge Cambridgeshire United Kingdom CB2 1GA Dendooven T Sinha D Roeselová A Cameron TA De Lay NR Luisi BF Bandyra KJ National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM121368 United States 6.8 10.6019/EMPIAR-12067 EMDB molecule EMD-12884 Dendooven T Sinha D Roeselová A Cameron TA De Lay NR Luisi BF Bandyra KJ A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation Molecular cell Mol Cell United Kingdom 14 81 2901 2913.e5 2021 English 10.1016/j.molcel.2021.05.032 34157309

Unaligned micrographs of the PNPase-3'ETS(LeuZ)-Hfq complex.

A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation /data/PNP_ETS_HFQ micrographs - multiframe TIFF TIFF 6271 38 UNSIGNED 32 BIT INTEGER 3838 1.065 3710 1.065

PNPase-3'ETS(LeuZ)_Hfq present in micrographs. The images were taken using an FEI Titan Krios, 300kV, with an average electron dose per image of 53.6 electrons per square Angstrom. Defocus -1 to -2.5um.