REL 2024-04-19 2024-07-04 2024-11-13 0000-0003-3976-0843 Shotaro Otsuka Max Perutz Labs Dr. Bohr Gasse 9 Vienna Austria 1030 0000-0003-3976-0843 Shotaro Otsuka Max Perutz Labs Vienna Austria 1030 Bragulat-Teixidor H Otsuka S Vienna Science and Technology Fund (WWTF) LS19-001 Austria Austrian Science Fund P 36743-B Austria 304.4 10.6019/EMPIAR-12050 EMDB cell EMD-50115 Bragulat-Teixidor H Ishihara K Szücs GM Otsuka S EMBO reports EMBO Rep 2024 English 10.1038/s44319-024-00175-w 38877171 Tilt series of ER-ER junctions of HeLa cells in interphase /data/data/TiltSeries tilt series PNG TIFF 110 1 SIGNED 16 BIT INTEGER 4096 4.51 4096 4.51

HeLa cells were instantly frozen using a high-pressure freezing machine (High Pressure Freezer Leica EM ICE equipped with the Coral Life workflow; Leica Microsystems) and freeze-substituted into Agar 100 Epoxy resin (Agar Scientific). As fiducial markers, 15 nm of gold-conjugated Protein A (Cytodiagnostics, AC-15-05-10) was absorbed on both sides of 250 nm sections. Sections were post-stained with 2% UA and lead citrate. Dual axis tilt series were acquired with a Tecnai G2 20 transmission electron microscope operated at 200 kV and equipped with an Eagle 4k HS CCD camera (FEI) by using the Serial EM software. Images were recorded over a -55° to 55° tilt range on average with an angular increment 1°.

Reconstructed tomograms of ER-ER junctions of HeLa cells in interphase /data/data/Tomograms reconstructed volumes PNG MRC 110 1 SIGNED 16 BIT INTEGER 4096 4.51 4096 4.51