REL
2024-01-23
2025-06-30
2025-06-30
Cryo-Electron Tomography Data and Comprehensive Annotation of C. elegans Centrosomes in Interphase and Mitosis
0000-0001-6968-041X
Julia
Mahamid
EMBL
Meyerhofstrasse 1
Heidelberg
Baden-Wuerttemberg
Germany
69117
Julia
Mahamid
EMBL
Meyerhofstrasse 1
Heidelberg
Baden-Wuerttemberg
Germany
69117
Tollervey F
European Research Council (ERC)
760067
Germany
111.4
10.6019/EMPIAR-12049
EMDB
cell
EMD-19778
EMD-19779
EMD-19780
EMD-19781
Tollervey F.
Rios M.U.
Zagoriy I.
Woodruff J.B.
Mahamid J.
Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography
10.1016/j.devcel.2024.12.002
Raw reconstructed tomograms of C. elegans centrosomes
/data/rawTomos
reconstructed volumes
MRC
MRC
12
1
UNSIGNED 32 BIT INTEGER
980
13.4808
928
13.4808
Tomograms aligned in etomo and reconstructed in warp using a back projection at 4x binning. A combination of patch tracking and fiducials were used depending on the tomogram
data/labels
Segmentations of microtubules, membranes, ribosomes, centrioles, and centrosomal matrix.
MRC
Unaligned multi-frame micrographs of C. elegans centrosomes
/data/frames
micrographs - multiframe
TIFF
TIFF
681
8
8
14
UNSIGNED 16 BIT INTEGER
3838
3.37
3710
3.37
Raw unaligned frame of C. elegans centrosomes, including metadata (modified and unmodified mdoc files), and alignment data (.xf, .rawtilt, and .st files). Images consist of 8 to 14 frames depending on the image set. To relate image set to centrosome number as seen in the label and tomogram directories, please refer to the readme file.
data/frames/*/*.tif