REL
2024-04-09
2024-06-17
2024-06-17
Bacterial selenocysteine synthase structure revealed by single-particle cryoEM
0000-0002-9398-2941
Vitor Hugo
Balasco Serrao
Biomolecular Cryoelectron Microscopy Facility, University of California - Santa Cruz
1156 High St
Santa Cruz
California
United States
95064
0000-0002-9398-2941
Vitor Hugo
Serrão
UCSC Biomolecular cryoEM facility
1156 High St
Santa Cruz
California
United States
95064
Balasco Serrao VH
Minari K
Pereira HM
Thiemann OH
National Institutes of Health (NIH)
S10OD02509
United States
Sao Paulo Research Foundation (FAPESP)
12/23730-1
Brazil
Brazilian National Council for Scientific and Technological Development (CNPq)
232251/2014-2, 140636/2013-7 and 479595/2006-2
Brazil
24.6
10.6019/EMPIAR-12043
EMDB
molecule
EMD-42845
Balasco Serrão VH
Minari K
Pereira HD
Thiemann OH
Bacterial selenocysteine synthase structure revealed by single-particle cryoEM
Current research in structural biology
Curr Res Struct Biol
United States
7
2024
English
10.1016/j.crstbi.2024.100143
38681238
Data collection was performed on a Titan Krios G3i at 300 kV equipped with a Gatan K3 direct detector coupled to a Bioquantum energy filter at 105,000X at a super-resolution counting mode with a physical pixel size of 0.826 Å/pix (super-res 0.413 Å/pix). A total of 4,592 movies were collected in a stack of 60 frames, and a total dose of 45.4 e−/Å2.
Unaligned multi-frames for apo-SelA
/data/processing/processing/P555
picked particles - multiframe - processed
TIFF
TIFF
4592
50
UNSIGNED BYTE
variable
variable
variable
variable