Cryo-EM Structures of human telomerase with TPP1-POT1 and telomerase H/ACA RNP

REL 2023-11-29 2024-04-22 2024-04-22 Cryo-EM Structures of human telomerase with TPP1-POT1 and telomerase H/ACA RNP 0000-0002-2180-3430 Thi Hoang Duong Nguyen MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0002-2180-3430 Thi Hoang Duong Nguyen MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH Ghanim GEG Sekne ZS Balch SB M. van Roon MVR Nguyen THDN UK Research and Innovation (UKRI) MC_UP_1201/19 United Kingdom Jane Coffin Childs (JCC) Fund na United States European Molecular Biology Organization (EMBO) na United Kingdom 13.2 10.6019/EMPIAR-11985 EMDB molecule EMD-17190 EMD-17191 EMD-14196 EMD-14197 EMD-14198 EMD-14199 Ghanim GE Sekne Z Balch S M. van Roon AM Nguyen THD 2.7 Å cryo-EM structure of human telomerase H/ACA ribonucleoprotein Nature communications Nat Commun 1 15 2024 English 10.1038/s41467-024-45002-x 38272871 Sekne Z Ghanim GE M. van Roon AM Nguyen THD Structural basis of human telomerase recruitment by TPP1-POT1 Science (New York, N.Y.) Science 6585 375 1173 1176 2022 English 10.1126/science.abn6840 35201900 Dataset 1 of unaligned multi-frame micrographs of the human Telomerase-DNA-TPP1-POT1 complex. /data/Dataset_1 micrographs - multiframe TIFF TIFF 4912 48 UNSIGNED BYTE 5760 1.059 4092 1.059

Dataset 2 of unaligned multi-frame micrographs of the human Telomerase-DNA-TPP1-POT1 complex. /data/Dataset_2 micrographs - multiframe TIFF TIFF 36141 48 UNSIGNED BYTE 5760 1.059 4092 1.059

Dataset 3 of unaligned multi-frame micrographs of the human Telomerase-DNA-TPP1-POT1 complex. /data/Dataset_3 micrographs - multiframe TIFF TIFF 9722 48 UNSIGNED BYTE 5760 1.11 4092 1.11

Human telomerase-DNA-TPP1 complex particle stack ( EMD-14196) /data/ParticleStacks/EMD-14196 picked particles - single frame - processed MRCS MRC 144043 1 16 BIT FLOAT 280 variable 280 variable

Human telomerase-DNA-TPP1-POT1 complex particle stack ( EMD-14197) /data/ParticleStacks/EMD-14197 picked particles - single frame - processed MRCS MRC 192871 1 16 BIT FLOAT 280 variable 280 variable

Human telomerase H/ACA RNP lobe of with the dyskerin thumb loop in an "open" conformation particle stack ( EMD-17191) /data/ParticleStacks/EMD-17191 picked particles - single frame - processed MRCS MRC 145627 1 16 BIT FLOAT 280 variable 280 variable

Human telomerase H/ACA RNP lobe of with the dyskerin thumb loop in an “closed” conformation particle stack ( EMD-17190) /data/ParticleStacks/EMD-17190 picked particles - single frame - processed MRCS MRC 199360 1 16 BIT FLOAT 280 variable 280 variable