REL
2023-12-11
2024-06-11
2024-06-11
Polymerase module of CPF in complex with Mpe1 and a pre-cleaved CYC1 RNA
0000-0003-1815-3710
Lori
A
Passmore
MRC Laboratory of Molecular Biology
Francis Crick Ave
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0003-1815-3710
Lori
A
Passmore
MRC Laboratory of Molecular Biology
Francis Crick Ave
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Rodríguez-Molina JB
O'Reilly FJ
Fagarasan H
Sheekey E
Maslen S
Skehel JM
Rappsilber J
Passmore LA
Medical Research Council (MRC, United Kingdom)
MC_U105192715
United Kingdom
European Research Council (ERC)
725685
United Kingdom
2.3
10.6019/EMPIAR-11962
EMDB
molecule
EMD-14710
Rodríguez-Molina JB
O'Reilly FJ
Fagarasan H
Sheekey E
Maslen S
Skehel JM
Rappsilber J
Passmore LA
Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF
Molecular cell
Mol Cell
13
82
2022
English
10.1016/j.molcel.2022.04.021
35584695
For this complex 11,856 movies were collected on Krios II at eBIC with a K3 detector in counting mode (bin 1), pixel size of 0.83 Å/pixel, total dose of 40 e-/Å^2 with a defocus range of -0.5 μm to -3.1 μm in 0.2 μm steps.
multi-frame micrographs of the polymerase module of CPF in complex with Mpe1 and a pre-cleaved CYC1 RNA
/data/14092020_JR20-1_PAM-Mpe1-42mer_eBIC_bi23268_Krios2_K3_105kx_40TD/raw
micrographs - multiframe
MRC
TIFF
11856
50
UNSIGNED BYTE
5760
0.83
4092
0.83