REL 2024-02-22 2024-08-23 2024-11-19 0000-0002-7191-0621 William C Earnshaw Wellcome Centre for Cell Biology, University of Edinburgh Max Born Crescent Edinburgh United Kingdom EH9 3BF 0000-0002-7191-0621 William C Earnshaw Wellcome Centre for Cell Biology, University of Edinburgh Max Born Crescent Edinburgh United Kingdom EH9 3BF Cisneros- Soberanis F Beckett AJ Simpson E Pucekova N Prior IA Booth DG Earnshaw WC Wellcome Trust 107022 United Kingdom Cancer Research UK CR1166 United Kingdom 31.6 10.6019/EMPIAR-11953 SBF-SEM cell Cisneros-Soberanis F Simpson EL Beckett AJ Pucekova N Corless S Kochanova NY Prior IA Booth DG Earnshaw WC The Journal of cell biology J Cell Biol United Kingdom 11 223 2024 English 10.1083/jcb.202403165 39186086 Anaphase 1 /data/Anaphase 1/55min_04_TIFF_5X5_8bit_corr (28x28x90) micrographs - single frame TIFF TIFF 199 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 28X28X90 nm

data/Anaphase 1/AMIRA files Chromosome segmentation AM data/Anaphase 1/55min_04_TIFF_5X5_8bit_corr (28x28x90)/55min_04_0000.tif Anaphase 2 /data/Anaphase 2/55min_07_TIFF_8bit_5X5 micrographs - single frame TIFF TIFF 182 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 27X27X80 nm

data/Anaphase 2/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Anaphase 2/55min_07_TIFF_8bit_5X5/55min_07_8bit_5X5_0000.tif Anaphase 3 /data/Anaphase 3/Anaphase 9I_TIFF_5X5_8bit micrographs - single frame TIFF TIFF 295 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 36X36X80 nm

data/Anaphase 3/Anaphase 9I - AMIRA Files Chromosome segmentation and Chromosome separation (ID) AM data/Anaphase 3/Anaphase 9I_TIFF_5X5_8bit/9I_5X5_tiffs0000.tif Anaphase 4 /data/Anaphase 4/5N (Original Files) micrographs - single frame DM4 DM4 309 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

Raw data

data/Anaphase 4/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Anaphase 4/5N (Original Files)/5N_000.dm4 Prometaphase 1 /data/Early prometaphase 1/TIFF 8X8 28.8X28.8X70 micrographs - single frame TIFF TIFF 164 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 29X29X70 nm

data/Early prometaphase 1/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Early prometaphase 1/TIFF 8X8 28.8X28.8X70/EM4 1A 28.8X28.8X70_0000.tif Prometaphase 2 /data/Early prometaphase 2/Prophase (EM5 3A)_TIFF_enh5_8bit_28X28X70 micrographs - single frame TIFF TIFF 150 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 28X28X70 nm

data/Early prometaphase 2/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Early prometaphase 2/Prophase (EM5 3A)_TIFF_enh5_8bit_28X28X70/Prophase (EM5 3A)_TIFF_enh5_8bit_28X28X700000.tif Metaphase 1 /data/Metaphase 1 5X5 (40min_Cell1)/40min-Cell1 (Original files) micrographs - single frame DM4 DM4 250 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

Raw data

data/Metaphase 1 5X5 (40min_Cell1)/40min_Cell1- AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Metaphase 1 5X5 (40min_Cell1)/40min-Cell1 (Original files)/40_001.dm4 Metaphase 2 /data/Metaphase 4 5X5 (Metaphase 1a)/Metaphase1a_TIFF_5X5_8bit micrographs - single frame TIFF TIFF 225 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 31X31X75 nm

data/Metaphase 4 5X5 (Metaphase 1a)/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Metaphase 4 5X5 (Metaphase 1a)/Metaphase1a_TIFF_5X5_8bit/Metaphase1_5X5_TIFFenh0000.tif Metaphase 3 /data/Metaphase 7 5X5 (Metaphase 3a) micrographs - single frame TIFF TIFF 231 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 28X28X75 nm

data/Metaphase 7 5X5 (Metaphase 3a)/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Metaphase 7 5X5 (Metaphase 3a)/Metaphase3a.tif Prometaphase 3 /data/Prometaphase 3/50min (Origina files) micrographs - single frame DM4 DM4 281 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

Raw data

data/Prometaphase 3/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Prometaphase 3/50min (Origina files)/50_000.dm4 Prometaphase 4 /data/Prometaphase 4 micrographs - single frame TIFF TIFF 309 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 3X3X75 nm

data/Prometaphase 4 Chromosome segmentation and Chromosome separation (ID) AM data/Prometaphase 4/Prometaphase1_TIFF.tif Telophase 1 /data/Telophase 1 micrographs - single frame TIFF TIFF 203 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 13X13X80 nm

data/Telophase 1/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Telophase 1/S6_TIFF.tif Telophase 2 /data/Telophase 2/55min_05 (70X70X65) micrographs - single frame TIFF TIFF 200 1 SIGNED BYTE variable variable variable variable

TIFF files Type: 8-bit Voxel size: 70X70X65 nm

data/Telophase 2/AMIRA files Chromosome segmentation and Chromosome separation (ID) AM data/Telophase 2/55min_05 (70X70X65)/55min_05_0054.tif