Single-particle cryo-EM dataset of the glycosyltransferase ArnC from Salmonella enterica in the apo state from Talos Arctica microscope

REL 2023-03-17 2025-06-13 2025-06-13 Single-particle cryo-EM dataset of the glycosyltransferase ArnC from Salmonella enterica in the apo state from Talos Arctica microscope 0000-0002-3194-9863 Vasileios I Petrou Rutgers New Jersey Medical School 225 Warren Street Newark NJ United States 07103 0000-0002-3194-9863 Vasileios I Petrou Rutgers New Jersey Medical School 225 Warren Street Newark NJ United States 07103 Ashraf KU Punetha A Kaelber JT Petrou VI National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R00GM123228 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM150831 United States 1.3 10.6019/EMPIAR-11924 EMDB molecule EMD-43617 Ashraf KU Bunoro-Batista M Ansell TB Punetha A Rosario-Garrido S Firlar E Kaelber JT Stansfeld PJ Petrou VI Structural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteria bioRxiv bioRxiv United States 2025 English 10.1101/2025.01.29.634835

The single-particle cryo-EM dataset contains unaligned movies (35 frames per movie, accumulated exposure of 35.21 electrons per angstrom squared, gain corrected), patch-aligned/doseweighted micrographs (subset contributing to final particle stack) and final particle stack used for reconstruction deposited in EMDB (EMD-43617).

Unaligned multi-frame micrographs of ArnC from S. enterica in the apo state collected on Talos Arctica /data/rawdata micrographs - multiframe TIFF TIFF 5552 35 1 35 UNSIGNED 32 BIT INTEGER 3838 1.038 3710 1.038

The dataset contains unaligned gain-corrected micrograph movies in TIFF format. Cryo-EM data were collected at the Rutgers Cryo-EM & Nanoimaging Facility using a 200 kV Talos Arctica (FEI/ThermoFisher) electron microscope equipped with a K2 Summit direct electron detector (Gatan) and a BioQuantum energy filter (Gatan) with slit width of 20 eV. Data were collected automatically in counting mode using EPU (FEI/ThermoFisher), a nominal magnification of 130,000X, a nominal pixel size of 1.038 Å/pixel, and a dose rate of 5.420 electrons/pixel/s. Movies were recorded at 200 ms/frame for 7s (35 frames total), resulting in a total radiation dose of 35.21 electrons/Å2. Nominal defocus range was -0.5 to -2.5 μm. A total of 5,552 micrographs were recorded from one grid over three days. Micrographs were gain-normalized and defect corrected. At the end of processing a calibrated pixel size of 1.05 Å/pixel was determined for this dataset, representing an 1.16% error from the nominal pixel size value.

data/rawdata/KA05_2*.tif Aligned and dose-weighted micrographs of ArnC from S. enterica in the apo state collected on Talos Arctica /data/aligned micrographs - single frame MRC MRC 3318 1 32 BIT FLOAT 3838 1.038 3710 1.038

Aligned and dose-weighted micrographs produced from the raw movies using patch motion correction in cryoSPARC. This dataset only contains a subset of micrographs that have contributed particles to the final particle stack. Nominal pixel size 1.038 Å/pixel. Calibrated pixel size imposed on final reconstruction is 1.05 Å/pixel.

data/aligned/*patch_aligned_doseweighted.mrc Final particle stack of ArnC from S. enterica in the apo state collected on Talos Arctica /data/particles picked particles - single frame - processed MRC MRC 3318 1 32 BIT FLOAT 288 1.038 288 1.038

Final particle stack of 184,679 from 3318 aligned/dose-weighted micrographs corresponding to the reconstruction deposited in EMD-43617. Nominal pixel size 1.038 Å/pixel. Calibrated pixel size imposed on final reconstruction is 1.05 Å/pixel.

data/particles/extract/*patch_aligned_doseweighted_particles.mrc data/particles/J278_particles_exported.cs