REL 2024-01-26 2024-07-12 2024-07-12 0000-0001-7835-6005 Linda Makhlouf Astbury Centre for Structural Molecular Biology, University of Leeds Leeds West Yorkshire United Kingdom LS2 9JT 0000-0003-0239-5926 Elton Zeqiraj Astbury Centre for Structural Molecular Biology, University of Leeds Leeds West Yorkshire United Kingdom LS2 9JT Yogesh Kulathu MRC Protein Phosphorylation & Ubiquitylation Unit, University of Dundee Dow Street Dundee United Kingdom DD1 5EH Makhlouf L Wellcome Trust 222531/Z/21/Z United Kingdom Wellcome Trust 222372/Z/21/Z United Kingdom Biotechnology and Biological Sciences Research Council (BBSRC) BB/T008172/1 United Kingdom European Research Council (ERC) 677623 United Kingdom Medical Research Council (MRC, United Kingdom) MC_UU_00018/3 United Kingdom Wellcome Trust 221524/Z/20/Z United Kingdom 22.6 10.6019/EMPIAR-11906 EMDB molecule EMD-18381 EMD-18382 Makhlouf L Peter JJ Magnussen HM Thakur R Millrine D Minshull TC Harrison G Varghese J Lamoliatte F Foglizzo M Macartney T Calabrese AN Zeqiraj E Kulathu Y Nature Nature 8003 627 437 444 2024 English 10.1038/s41586-024-07093-w 38383789 Unaligned multiframe micrographs in EER format of the UFM1 ribosome E3 ligase complex bound to the 60S ribosome subunit /data/Images-Disc1 micrographs - multiframe EER EER 59394 819 UNSIGNED 16 BIT INTEGER variable 0.74 variable 0.74

Dose per physical pixel/s of 6.85 used resulting in a total dose of 33.4 e/Å2. Defocus range of -0.2 to -2. For motion correction, 819 internal frames were grouped by 20 resulting in 42 EER fractions with a dose per frame of 0.8 e/A2. Gain reference file called "20221115_154105_EER_GainReference.gain".

Aligned micrographs of the UFM1 ribosome E3 ligase complex bound to the 60S ribosome subunit /data/job005/Raw_data micrographs - single frame MRC MRC 59394 1 32 BIT FLOAT variable variable variable variable

Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 20 and dose per frame of 0.8 e/A2. 5, 5 patches were used.