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        <keyDates>
            <depositionDate>2024-02-10</depositionDate>
            <releaseDate>2025-04-24</releaseDate>
            <updateDate>2025-04-24</updateDate>
        </keyDates>
        <title>Single particle cryo-EM of ASCT2 in lipid nanodiscs under Na+-depleted conditions</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-7017-109X</authorORCID>
            <firstName>Cristina</firstName>
            <lastName>Paulino</lastName>
            <organization type="academic">University of Heidelberg, Biochemistry Center (BZH)</organization>
            <street>Im Neuenheimer Feld 328</street>
            <townOrCity>Heidelberg</townOrCity>
            <country>Germany</country>
            <postOrZipCode>69120</postOrZipCode>
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            <firstName>Cristina</firstName>
            <lastName>Paulino</lastName>
            <organization type="academic">University of Heidelberg, Biochemistry Center (BZH)</organization>
            <street>Im Neuenheimer Feld 328</street>
            <townOrCity>Heidelberg</townOrCity>
            <country>Germany</country>
            <postOrZipCode>69120</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-5804-9689</authorORCID>
            <firstName>Dirk-Jan</firstName>
            <lastName>Slotboom</lastName>
            <organization type="academic">University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute</organization>
            <street>Nijenborgh 4</street>
            <townOrCity>Groningen</townOrCity>
            <country>Netherlands</country>
            <postOrZipCode>9747 AG</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-4127-3497">Borowska AM</author>
            <author authorORCID="0000-0002-9901-2065">Rheinberger J</author>
            <author authorORCID="0000-0001-7017-109X">Paulino C</author>
            <author authorORCID="0000-0002-5804-9689">Slotboom DJ</author>
        </authorsList>
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                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>722.017.001</code>
                <country>Netherlands</country>
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            <grantReference>
                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>40.018.016</code>
                <country>Netherlands</country>
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        <entryDOI>10.6019/EMPIAR-11905</entryDOI>
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        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-18627</emdbEntry>
            <emdbEntry>EMD-18628</emdbEntry>
        </relatedEMDBEntries>
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            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-4127-3497" order="1">Borowska AM</author>
                    <author authorORCID="0000-0003-1076-682X" order="2">Chiariello MG</author>
                    <author authorORCID="0000-0002-7394-8981" order="3">Garaeva AA</author>
                    <author authorORCID="0000-0002-9901-2065" order="4">Rheinberger J</author>
                    <author authorORCID="0000-0001-8423-5277" order="5">Marrink SJ</author>
                    <author authorORCID="0000-0001-7017-109X" order="6">Paulino C</author>
                    <author authorORCID="0000-0002-5804-9689" order="7">Slotboom DJ</author>
                    <title>Structural basis of the obligatory exchange mode of human neutral amino acid transporter ASCT2</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>15</volume>
                    <year>2024</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-024-50888-8</externalReferences>
                    <externalReferences type="pubmed">39095408</externalReferences>
                </journalCitation>
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        </citationList>
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    <imageSet>
        <name>Unaligned movie frames of ASCT2 in lipid nanodiscs under Na+-depleted conditions (dataset 1)</name>
        <directory>/data/raw_220623_ASCT2-2N2-KCl_bAB7s3_ngc</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1633</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.022</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.022</pixelHeight>
        </dimensions>
        <details>Data represents unaligned multi-frame movies. Each movie contains 60 frames collected from the Quantifoil grid at 200 kV in counting mode on a K2 camera. Total accumulated electron exposure on the specimen level corresponds to 51 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.</details>
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    <imageSet>
        <name>Unaligned movie frames of ASCT2 in lipid nanodiscs under Na+-depleted conditions (dataset 2)</name>
        <directory>/data/raw_220628_ASCT2-2N2-KCl_bAB7s5_ngc</directory>
        <category>micrographs - multiframe</category>
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        <details>Data represents unaligned multi-frame movies. Each movie contains 60 frames collected from the Quantifoil grid at 200 kV in counting mode on a K2 camera. Total accumulated electron exposure on the specimen level corresponds to 51 electrons per Å^2. Movies are not gain-corrected, and the gain reference is provided. Data is LZW-compressed.</details>
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