REL 2024-02-12 2024-05-16 2024-05-16 0000-0002-5457-4478 Beata Turoňová Max Planck Institute of Biophysics Max-von-Laue-Strasse 3 Frankfurt am Main Hessen Germany 60438 0000-0002-5457-4478 Beata Turoňová Max Planck Institute of Biophysics, Department of Molecular Sociology Max-von-Laue-Strasse 3 Frankfurt am Main Hessen Germany 60438 0000-0002-7397-1321 Martin Beck Max Planck Institute of Biophysics, Department of Molecular Sociology Max-von-Laue-Strasse 3 Frankfurt am Main Hessen Germany 60438 0000-0001-7768-746X Gerhard Hummer Max Planck Institute of Biophysics, Department of Theoreotical Biophysics Max-von-Laue-Strasse 3 Frankfurt am Main Hessen Germany 60438 Tuijtel MW Kreysing JP Welsch S Hummer G Beck M Turoňová B Cruz-León S Chan Zuckerberg Initiative 2021-234666 United States 382.6 10.6019/EMPIAR-11899 EMDB cell EMD-19136 Tuijtel MW Cruz-León S Kreysing JP Welsch S Hummer G Beck M Turoňová B Science advances Sci Adv 17 10 2024 English 10.1126/sciadv.adk6285 38669330 Aligned tilt-series of Dictyostelium discoideum cells /data tilt series MRC MRC 213 61 17 61 UNSIGNED 16 BIT INTEGER 4096 1.223 4096 1.223

Tilt-series were aimed to collect 61 tilts, for some fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from +60 to -60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided. Tilts that were discarded during processing are specified in "tilt_cleaning.txt". Alignment results from IMOD are provided as _dose-filt.xf files. Coordinates to raw ribosome particle picks by template matching are provided in TM_SG_bin6_subtomos_3.1star, with coordinates with bin-factor of 6 corresponding to a pixel size of 7.3 Angstrom. Ribosome particle locations after 3D-classification are provided in Classification_relion_bin6_subtomos_3.1.star, with coordinates with bin-factor of 6 corresponding to a pixel size of 7.3 Angstrom.