REL
2024-02-08
2024-04-12
2024-04-12
Cryo-electron tomography data of Emiliania huxleyi virus 201 for subtomogram averaging
0000-0003-4215-3315
Pavel
Plevka
Central European Institute of Technology (CEITEC) Masaryk University
Kamenice 753/5
Brno
Czech Republic
62500
0000-0003-4215-3315
Pavel
Plevka
Central European Institute of Technology (CEITEC) Masaryk University
Kamenice 753/5
Brno
Czech Republic
62500
Homola M
Büttner CR
Füzik T
Nováček J
Chaillet M
Förster F
Plevka P
European Research Council (ERC)
101043454
Czech Republic
Grant Agency of the Czech Republic
GX19-25982X
Czech Republic
294.8
10.6019/EMPIAR-11896
EMDB
virus
EMD-17649
Homola M
Büttner CR
Füzik T
Nováček J
Chaillet M
Förster F
Plevka P
Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi
Science Advances
Sci. Adv.
United States
2024
English
10.1101/2023.06.30.547180
Information about the microscope settings and acquisition details are listed in the EMDB entry 17649.
Raw multiframes movies of EhV-201 particles tilt-series
/data/Movies
tilt series
TIFF
TIFF
283
33
0
48
BIT
5760
2.08
4092
2.08
Dataset contains 283 tilt-series, each consisting of 33 tilts (+/- 48 deg with 3 deg increment), each containing 6 frames. Dataset details: Titan Krios G2 (Thermofisher Scientific) 300 kV; detector K3 with Bioquantum energy filter (Gatan); Pixel size: 2.08; Total dose: 80 e/A^2; Dose-symmetric collection scheme. Data are not motion-corrected nor gain-corrected. Each tilt "fileName.tif" is accompanied by "fileName.tif.mdoc" file with acquisition details.
Gain file: data/Gain
mdoc tomo files: data/mdoc (to assemble the tilt-series from the individual tilts)
More information about the sample preparation and data acquisition can be found in the article (Homola et al. 2024 - Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi) or in the EMDB deposition 17649.