REL 2024-01-24 2024-02-06 2024-02-06 0000-0002-7601-210X Martin Jinek Department of Biochemistry, University of Zurich Winterthurerstrasse 190 Zurich ZH Switzerland 8057 0000-0002-7601-210X Martin Jinek Department of Biochemistry, University of Zurich Winterthurerstrasse 190 Zurich ZH Switzerland 8057 0000-0002-9576-3267 Ruedi Aebersold Department of Biology, Institute of Molecular Systems Biology, ETH Zurich Zurich ZH Switzerland 8093 Muckenfuss LM Jinek M European Research Council (ERC) ERC-StG-337284 Switzerland European Molecular Biology Organization (EMBO) EMBO ALTF-343-2013 Switzerland European Union (EU) PROSPECTS, HEALTH-F4-2008-201648 Switzerland European Research Council (ERC) ERC-StG-233226 Switzerland European Research Council (ERC) ERC-StG-670821 Switzerland 1.2 10.6019/EMPIAR-11875 EMDB molecule EMD-4225 Clerici M Faini M Muckenfuss LM Aebersold R Jinek M Nature structural & molecular biology Nat Struct Mol Biol 2 25 135 138 2018 English 10.1038/s41594-017-0020-6 29358758 Unaligned multi-frame micrographs of human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif /data/raw micrographs - multiframe TIFF TIFF 1 50 1 50 UNSIGNED BYTE 7676 1.058 7420 1.058

FEI Titan Krios microscope operated at 300 kV and equipped with a Gatan K2 Summit detector and a Gatan Quantum GIF LS energy filter. Micrographs were collected in super-resolution mode and fourier-cropped on-the-fly with FOCUS software, consisted of 50 frames for a total exposure time of 10 s and a total dose of 80 e-/Å2. The defocus ranged between –2.2 and –1 μm. Data collected with Serial EM.

data/raw/*.tif Gain reference /data/gainref micrographs - single frame DM4 DM4 1 1 32 BIT FLOAT 7676 1.058 7420 1.058

Gain reference

data/gainref/SuperRef_MC6-6_24_Oct18_18.35.07.dm4