REL 2024-01-20 2024-06-18 2024-06-18 β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after addition of GTP 0000-0003-0238-7846 Georgios Skiniotis Stanford University Stanford California United States 94305 0000-0003-3238-3213 Makaía M. Papasergi-Scott Stanford University Stanford California United States 94305 Papasergi-Scott MM Skiniotis G National Institutes of Health (NIH) K99HL16140601 United States National Institutes of Health (NIH) R01GM083118 United States 12.6 10.6019/EMPIAR-11857 EMDB molecule EMD-40116 EMD-40117 EMD-40118 EMD-40119 EMD-40120 EMD-40121 EMD-40122 EMD-40123 EMD-40124 EMD-40125 EMD-40126 EMD-40127 EMD-40128 EMD-40129 EMD-40130 EMD-40131 EMD-40132 EMD-40133 EMD-40134 EMD-40135 EMD-29985 EMD-29986 EMD-29987 EMD-29988 EMD-29989 EMD-29990 EMD-29991 EMD-29992 EMD-29993 EMD-29994 EMD-29995 EMD-29996 EMD-29997 EMD-29998 EMD-29999 EMD-40000 EMD-40001 EMD-40002 EMD-40004 EMD-40005 EMD-40009 EMD-40010 EMD-40011 EMD-40012 EMD-40013 EMD-40014 EMD-40015 EMD-40016 EMD-40017 EMD-40018 EMD-40019 EMD-40020 EMD-40021 EMD-40022 EMD-40023 EMD-40024 EMD-40025 EMD-40026 EMD-40027 EMD-40028 EMD-40156 EMD-40157 EMD-40158 EMD-40159 EMD-40160 EMD-40161 EMD-40163 EMD-40164 EMD-40165 EMD-40166 EMD-40167 EMD-40168 EMD-40169 EMD-40170 EMD-40171 EMD-40172 EMD-40173 EMD-40174 EMD-40175 EMD-40176 EMD-42408 EMD-42409 EMD-42410 EMD-42411 EMD-42412 EMD-42413 EMD-42414 EMD-42415 EMD-42416 EMD-42417 EMD-42418 EMD-42419 EMD-42420 EMD-42421 EMD-42422 EMD-42423 EMD-42424 EMD-42425 EMD-42426 EMD-42427 Papasergi-Scott MM Pérez-Hernández G Batebi H Gao Y Eskici G Seven AB Panova O Hilger D Casiraghi C He F Maul L Gmeiner P Kobilka BK Hildebrand PW Skiniotis G Skiniotis G Papasergi-Scott MM Time-resolved cryo-EM of G-protein activation by a GPCR Nature Nature United States 2024 English 10.1038/s41586-024-07153-1 38480881
Raw unaligned movie files (tif format, not gain corrected), and gain correction images (mrc format). Collected on a Titan Krios equipped with a K3 Summit direct electron detector. The microscope was operated at 300 kV accelerating voltage. Super-resolution pixel size is 0.42605Å.
 Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 1, Grid A) /data/Collection_1/Raw_Micrographs_Collection_1_Set_1 micrographs - multiframe TIFF TIFF 752 77 UNSIGNED BYTE 11520 0.42605 8184 0.42605
Raw unaligned movie files (tif format, 77 frames, not gain corrected). Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. movies were obtained at an exposure rate of 21.13 electrons/Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.
data/Collection_1/Raw_Micrographs_Collection_1_Set_1/20210923_MPS*.tif Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 1, Grid B) /data/Collection_1/Raw_Micrographs_Collection_1_Set_2 micrographs - multiframe TIFF TIFF 7258 77 UNSIGNED BYTE 11520 0.42605 8184 0.42605
Raw unaligned movie files (tif format, 77 frames, not gain corrected). Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. movies were obtained at an exposure rate of 21.13 electrons/Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.
data/Collection_1/Raw_Micrographs_Collection_1_Set_2/20210923_MPS*.tif Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 2, Grid B) /data/Collection_2 micrographs - multiframe TIFF TIFF 1949 77 UNSIGNED BYTE 11520 0.42605 8184 0.42605
Raw unaligned movie files (tif format, 77 frames, not gain corrected). Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. Movies were obtained at an exposure rate of 20.95 electrons/ Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.
data/Collection_2/20211220_MPS*.tif Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 3, Grid C) /data/Collection_3 micrographs - multiframe TIFF TIFF 11456 79 UNSIGNED BYTE 11520 0.42605 8184 0.42605
Raw unaligned movie files (tif format, 79 frames, not gain corrected). Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. Movies were obtained at an exposure rate of 30.71 electrons/Å^2/sec. The total exposure time was 2.008 sec over 79 frames per movie stack.
data/Collection_3/20211220_MPS*.tif