REL 2024-01-08 2024-02-15 2024-02-15 0000-0002-4159-6934 Eija Jokitalo Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki PL56 (Viikinkaari 9) Helsinki Uusimaa Finland 00014 0000-0002-4159-6934 Eija Jokitalo Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki PL56 (Viikinkaari 9) Helsinki Uusimaa Finland 00014 Belevich I Jokitalo E Academy of Finland 1331998 Finland 1.1 10.6019/EMPIAR-11849 FIB-SEM cell Belevich I Jokitalo E Localization of a Golgi matrix protein GM130 in human hepatocellular carcinoma cell (Huh-7) EMPIAR-11746 FIB-SEM dataset showing localization of a Golgi matrix protein GM130 (purple) in human hepatocellular carcinoma cell (Huh-7) /data/Images reconstructed volumes TIFF TIFF 564 1 UNSIGNED BYTE 3269 40.0 1397 40.0

Huh7, a human hepatoma-derived cell was immunostained using antibody against Golgi matrix protein GM130 and imaged using Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) with voxels of 4.0 x 4.0 x 8.0 nm. Immunolabeling as described in Lak B, Li S, Belevich I, Sree S, Butkovic R, Ikonen E, Jokitalo E. Specific subdomain localization of ER resident proteins and membrane contact sites resolved by electron microscopy. Eur J Cell Biol. 2021 Sep-Nov;100(7-8):151180. doi: 10.1016/j.ejcb.2021.151180. Epub 2021 Oct 4 Stained using ferrocyanide-reduced osmium + thiocarbohydrazide + osmium (OTO) method Purified mouse anti-GM130 was from BD biosciences.

data/Labels Segmentation model of gold particles of a human hepatoma-derived cell (Huh-7) immunostained using antibody against Golgi matrix protein GM130 and imaged using Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) with voxels of 4.0 x 4.0 x 8.0 nm Segmentation was done in DeepMIB using 54 images as a training set I. Belevich, and E. Jokitalo, DeepMIB: User-friendly and open-source software for training of deep learning network for biological image segmentation, PLoS Comput Biol. 2021 Mar 2;17(3):e1008374. doi: 10.1371/journal.pcbi.1008374 data/Images TIFF