REL
2023-08-25
2024-02-01
2024-02-01
Structure of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes
0000-0001-9132-4737
Martina
Foglizzo
Astbury Centre for Structural Molecular Biology, University of Leeds
Leeds
West Yorkshire
United Kingdom
LS2 9JT
0000-0001-9551-5514
Marcus
D
Wilson
Wellcome Centre for Cell Biology, University of Edinburgh
Edinburgh
United Kingdom
EH9 3JR
0000-0003-0239-5926
Elton
Zeqiraj
Astbury Centre for Structural Molecular Biology, University of Leeds
Leeds
United Kingdom
LS2 9JT
Foglizzo M
Burdett H
Wilson MD
Zeqiraj E
Medical Research Council (MRC, United Kingdom)
MR/T029471/1
United Kingdom
Wellcome Trust
222531/Z/21/Z
United Kingdom
Wellcome Trust
108466/Z/15/Z
United Kingdom
Wellcome Trust
221524/Z/20/Z
United Kingdom
10.4
10.6019/EMPIAR-11840
EMDB
molecule
EMD-16859
EMD-17928
Burdett H
Foglizzo M
Musgrove LJ
Kumar D
Clifford G
Campbell LJ
Heath GR
Zeqiraj E
Wilson MD
BRCA1-BARD1 combines multiple chromatin recognition modules to bridge nascent nucleosomes
Nucleic acids research
Nucleic Acids Res
United Kingdom
20
51
11080
11103
2023
English
10.1093/nar/gkad793
37823591
Unaligned multiframe movies in the EER format of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes
/data/24092021_RawData
micrographs - multiframe
EER
EER
16009
1204
UNSIGNED 16 BIT INTEGER
variable
0.86
variable
0.86
A dose per physical pixel/s of 5.38 was used, resulting in a total electron dose of 36.4 e-/Å2, fractionated across 172 EPU frames. These were then grouped into 26 frames, resulting in an electron dose of 0.8 e-/Å2 per frame.
data/24092021_RawData/Images-Disc1/GridSquare_*/Data/*.eer
Aligned micrographs of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes
/data/Raw_data
micrographs - single frame
MRC
MRC
16009
1
32 BIT FLOAT
variable
0.86
variable
0.86
Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 45.
data/Raw_data/*.mrc