REL 2023-12-07 2024-06-04 2024-06-04 0000-0002-4849-4148 Luke Chao Department of Molecular Biology, Massachusetts General Hospital 185 Cambridge Street Boston Massachusetts United States 02114 0000-0002-4849-4148 Luke Chao Department of Molecular Biology, Massachusetts General Hospital 185 Cambridge Street Boston Massachusetts United States 02114 Fry MY Navarro PP Ananda VY Ge Y McDonald JL Hakim P Luce BE Lugo CM Chao LH National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM142553 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) R01DK125263 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R37CA248565 United States Jane Coffin Childs (JCC) Fund 243493 United States Swiss National Science Foundation P2BSP3_188112 Switzerland Swiss National Science Foundation P400PB_199252 Switzerland 21.4 10.6019/EMPIAR-11816 EMDB cell EMD-43057 EMD-43056 EMD-43055 EMD-43054 EMD-43053 Fry MY Navarro PP Hakim P Ananda VY Qin X Landoni JC Rath S Inde Z Lugo CM Luce BE Ge Y McDonald JL Ali I Ha LL Kleinstiver BP Chan DC Sarosiek KA Chao LH The EMBO journal EMBO J 3 43 391 413 2024 English 10.1038/s44318-024-00027-2 38225406 Unaligned multi-frame movies of tilt-series of cryo-FIB milled Mouse Embryonic Fibroblasts with OPA1 deletion and stable expression of OPA1 isoform 5 /data/EMPIAR micrographs - multiframe TIFF TIFF 284 6 1 6 UNSIGNED BYTE 11520 1.375 8184 1.375

Unaligned multi-frame micrographs (EMD-430*/frames/*.tiff) corresponding to 5 tilt series with corresponding gain reference (gain/*dm4) and metadata (mdoc/*.mdoc). Data acquired on a K3 BioQuantum detector with no Volta phase plate. Acquisition parameters differ for each dataset (named after the corresponding EMDB accession code) and are specified below. EMD-43053: 63 tilts in the series. EMD-43054: 62 tilts in the series EMD-43055: 63 tilts in the series EMD-43056: 48 tilts in the series EMD-43057: 48 tilts in the series