CryoEM micrographs of a group II RNP in the post-ligation state

REL 2023-10-30 2024-07-14 2024-07-14 CryoEM micrographs of a group II RNP in the post-ligation state 0000-0001-9045-8872 Anna Marie Pyle Molecular, Cellular and Developmental Biology (MCDB), Yale University 260 Whitney Ave New Haven Connecticut United States 06511 0000-0001-9045-8872 Anna Marie Pyle Molecular, Cellular and Developmental Biology (MCDB), Yale University 260 Whitney Ave New Haven Connecticut United States 06511 Xu L Liu T Chung K Howard Hughes Medical Institute (HHMI) HHMI United States 5.9 10.6019/EMPIAR-11809 EMDB molecule EMD-40987 Xu L Liu T Chung K Pyle AM Chung K Structural insights into intron catalysis and dynamics during splicing Nature Nature United States 2023 English 10.1038/s41586-023-06746-6 37993708

For the Chameleon dataset (13 740 micrographs), a nominal magnification of ×105,000 and a defocus range of −1.0 to −2.5 μm was used, giving an effective pixel size of 0.832 Å at the specimen level. Each micrograph was dose-fractionated to 50 frames with a total exposure time of 1.5 s and a frame exposure time of 0.03 s, resulting in a total dose of 50.5 e−/Å2. For the Vitrobot dataset (3 872 micrographs), a nominal magnification of ×105,000 and a defocus range of −1.0 to −2.0 μm was used, giving an effective pixel size of 0.832 Å at the specimen level. Each micrograph was dose-fractionated to 48 frames with a total exposure time of 1.92 s and a frame exposure time of 0.04 s, resulting in a total dose of 50 e−/Å2.

Unaligned multi-frame micrographs of a group IIC intron (post-exon ligation) /data/Post_2F_RNP_1 micrographs - multiframe TIFF TIFF 13740 50 UNSIGNED BYTE 11520 0.832 8184 0.832

data/Post_2F_RNP_1/*.tif Unaligned multi-frame micrographs of a group IIC intron (post-exon ligation) /data/Post_2F_RNP_2 micrographs - multiframe TIFF TIFF 3872 48 UNSIGNED BYTE 11520 0.832 8184 0.832

data/Post_2F_RNP_2/*.tif