REL
2023-11-03
2025-11-21
2025-11-21
Single-particle cryo electron microscopy of Integrin a4b7 bound to MadCAM-1
0000-0003-1909-5751
Melody
Campbell
Basic Sciences Division, Fred Hutchinson Cancer Center
1100 Fairview Ave. N.
Seattle
Washington
United States
98109
0000-0003-3375-8937
Jeremy
Hollis
Basic Sciences Division, Fred Hutchinson Cancer Center
1100 Fairview Ave. N.
Seattle
Washington
United States
98109
Hollis JA
Campbell MG
National Institutes of Health (NIH)
R35GM147414
United States
3.5
10.6019/EMPIAR-11765
EMDB
molecule
EMD-71399
Hollis JA
Malik HS
Campbell MG
Chan MC
Molecular exaptation by the integrin αI domain
10.1126/sciadv.adx9567
Unaligned multi-frame movies of integrin a4b7 bound to MadCAM-1
/data
micrographs - multiframe
TIFF
TIFF
3223
100
UNSIGNED 16 BIT INTEGER
5760
0.561
4092
0.561
α4β7 integrin bound to MadCAM-1: 3223 unaligned uncorrected movies collected in two different datasets (803 + 2420) were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The movies contain 100 frames and the super resolution pixel size is 0.561 Å/px. The total dose per movie is ~50 e/Å^2. The gain reference is also included for each dataset in .dm4 format; 20220516_K3GainRefx1m3.dm4 is used to reference movies 20220517**.tif and 20220522_K3GainRefx1m3.dm4 is used to reference movies 20220525**.tif.
data/*.tif
Dose-weighted aligned micrographs of integrin a4b7 bound to MadCAM-1
/data
micrographs - single frame
MRC
MRC
3223
1
32 BIT FLOAT
5760
1.122
4092
1.122
α4β7 integrin bound to MadCAM-1: 3223 unaligned uncorrected movies collected over 2 datasets (803 + 2420) were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.
data/*.mrc
Dose-weighted aligned particle stacks of clasped apo-integrin a4b7 in the compact state
/data
picked particles - single frame - processed
MRCS
MRCS
188443
1
32 BIT FLOAT
364
1.122
364
1.122
Stacks of 188443 particles selected after sequential 2D and 3D classification contributing to final reconstruction of α4β7 integrin bound to MadCAM-1. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.
data/*.mrcs
data/*.star
data/