Cryo-EM SPA datasets of Streptococcus pneumoniae NADPH oxidase F397A mutant with NADPH under turnover conditions

REL 2023-10-16 2024-11-08 2024-11-08 Cryo-EM SPA datasets of Streptococcus pneumoniae NADPH oxidase F397A mutant with NADPH under turnover conditions 0000-0001-6341-9368 Bonnie J Murphy Redox and Metalloproteins, Max Planck Insitute of Physics Max-von-Laue-Strasse 3 Frankfurt am Main Hesse Germany 60438 0000-0001-6341-9368 Bonnie J Murphy Redox and Metalloproteins, Max Planck Insitute of Physics Max-von-Laue-Strasse 3 Frankfurt am Main Hesse Germany 60438 Dubach VRA San Segundo-Acosta P Murphy BJ Max Planck Society N/A Germany 16.6 10.6019/EMPIAR-11744 EMDB molecule EMD-18647 Dubach VRA San Segundo-Acosta P Murphy BJ Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase Nature structural & molecular biology Nat Struct Mol Biol 2024 English 10.1038/s41594-024-01348-w 39039317

Raw micrograph frames recorded in EER format with a total dose of 70 e/Å using a Falcon IV detector in counting mode on a 300 kV Titan Krios G4 microscope. A total of 924, 899, 1008 and 994 EER frames, respectively, were recorded and not gain corrected. Sample was frozen on a gold support grid (UltrAuFoil).

FUnaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4 /data/spNOX_F397A/dataset1 micrographs - multiframe EER EER 21895 924 BIT 4096 0.573 4096 0.573

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter. The EER movies contain 924 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV. Gain reference is provided.

data/spNOX_F397A/dataset1/movies_*/*.eer Unaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4 /data/spNOX_F397A/dataset2 micrographs - multiframe EER EER 9583 889 BIT 4096 0.573 4096 0.573

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter. The EER movies contain 889 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV. Gain reference is provided.

data/spNOX_F397A/dataset2/movies_*/*.eer Unaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4 /data/spNOX_F397A/dataset3 micrographs - multiframe EER EER 6332 1008 BIT 4096 0.573 4096 0.573

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter. The EER movies contain 1008 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV. Gain reference is provided.

data/spNOX_F397A/dataset3/movies_*/*.eer Unaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4 /data/spNOX_F397A/dataset4 micrographs - multiframe EER EER 7415 994 BIT 4096 0.573 4096 0.573

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter. The EER movies contain 994 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV. Gain reference is provided.

data/spNOX_F397A/dataset4/movies_*/*.eer