REL
2023-10-16
2025-06-26
2025-07-03
Cryo-EM SPA dataset of stably reduced Streptococcus pneumoniae NADPH oxidase with NADH
0000-0001-6341-9368
Bonnie
J
Murphy
Redox and Metalloproteins, Max Planck Insitute of Physics
Max-von-Laue-Strasse 3
Frankfurt am Main
Hesse
Germany
60438
0000-0001-6341-9368
Bonnie
J
Murphy
Redox and Metalloproteins, Max Planck Insitute of Physics
Max-von-Laue-Strasse 3
Frankfurt am Main
Hesse
Germany
60438
Dubach VRA
San Segundo-Acosta P
Murphy BJ
Max Planck Society
N/A
Germany
11.1
10.6019/EMPIAR-11743
EMDB
molecule
EMD-18646
Dubach VRA
San Segundo-Acosta P
Murphy BJ
Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase
Nature structural & molecular biology
Nat Struct Mol Biol
2024
English
10.1038/s41594-024-01348-w
39039317
Raw micrograph frames recorded in EER format with a total dose of 70 e/Å using a Falcon IV detector in counting mode on a 300 kV Titan Krios G4 microscope. A total of 938 EER frames were recorded and not gain corrected. Sample was frozen on a gold support grid (UltrAuFoil).
Unaligned mutliframe non-gain-normalized EER movies of SpNOX collected on a Krios G4
/data/spNOX_NADHbound_anaerobic
micrographs - multiframe
EER
EER
28120
938
BIT
4096
0.573
4096
0.573
Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.
The EER movies contain 938 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.
XML files to generate optics groups are provided as well as the gain reference.
data/spNOX_NADHbound_anaerobic/movies_*/*.eer