REL
2023-08-13
2024-08-22
2024-08-22
Substrate-free AAA single-chained ClpX∆N protein in a complex with ClpP peptidase
0000-0002-1719-5399
Alireza
Ghanbarpour
MIT
77 Massachusetts Ave.
Cambridge
MA
United States
02139
0000-0002-8858-8907
Joseph
H
Davis
MIT
77 Massachusetts Ave.
Cambridge
MA
United States
02139
0000-0002-1719-5399
Robert
T
Sauer
MIT
77 Massachusetts Ave.
Cambridge
MA
United States
02139
Ghanbarpour A
Cohen SE
Fei X
Davis JH
Sauer RT
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01-GM144542; R35-GM141517
United States
894.9
10.6019/EMPIAR-11707
EMDB
molecule
EMD-27946
Ghanbarpour A
Cohen SE
Fei X
Sauer RT
Davis JH
Davis JH
Sauer RT
A closed translocation channel in the substrate-free AAA+ ClpXP protease diminishes rogue degradation
Nature Communications
2023
English
10.1101/2022.08.27.505532
Ghanbarpour A
Cohen SE
Fei X
Bell TA
Baker TA
Davis JH
Sauer RT
Davis JH
Sauer RT
A closed translocation channel in the substrate-free AAA+ ClpXP protease diminishes rogue degradation
bioRxiv
2022
10.1101/2022.08.27.505532
Unaligned Multiframes Micrographs
/data
micrographs - multiframe
TIFF
TIFF
2405
30
1
30
UNSIGNED BYTE
8184
0.416
11520
0.416
Defocus range (min, max, step):-0.75, -2.5, -0.25
Total exposure on specimen: 49.39 el/A2
Objective lens spherical aberration (Cs): 2.7mm
Acceleration voltage: 300kV
uncalibrated pixel size: 0.435 Å
calibrated pixel size: 0.416 Å
Particle stacks of substrate-free ClpX(deltaN domain).ClpP (singly capped)
/data/Particles_EMD-27941
picked particles - single frame - processed
MRC
MRC
49824
1
32 BIT FLOAT
300
1.305
300
1.305
Final Particle stack for EMD-27941 (singly capped ClpX-deltaN.ClpP)
Particle stacks of substrate-free ClpX(delta-N domain).ClpP (doubly capped)
/data/Particles_EMD-27946
picked particles - single frame - processed
MRC
MRC
171869
1
32 BIT FLOAT
256
1.106
256
1.106
Final Particle stack for EMD-27946 (doubly capped ClpX-deltaN.ClpP)