Working on 278-1_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 24.2195 Std = 5.38447 Hotpixel threshold = 56.5263 Detected 285 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 5.15805 px Iteration 2: RMSD = 0.476495 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 3.626 px Iteration 2: RMSD = 0.529275 px Iteration 3: RMSD = 0.170396 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 2.19552 px Iteration 2: RMSD = 0.302208 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 2.12583 px Iteration 2: RMSD = 0.588448 px Iteration 3: RMSD = 0.182402 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 1.84968 px Iteration 2: RMSD = 0.532459 px Iteration 3: RMSD = 0.172132 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 2.33092 px Iteration 2: RMSD = 0.840336 px Iteration 3: RMSD = 0.58025 px Iteration 4: RMSD = 0.182413 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 2.26486 px Iteration 2: RMSD = 0.485793 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 2.09342 px Iteration 2: RMSD = 0.719431 px Iteration 3: RMSD = 0.119546 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 2.11401 px Iteration 2: RMSD = 0.406549 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 4.75719 px Iteration 2: RMSD = 0.526212 px Iteration 3: RMSD = 0.327907 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 2.05578 px Iteration 2: RMSD = 0.689322 px Iteration 3: RMSD = 0.279822 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 1.9908 px Iteration 2: RMSD = 0.454739 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 2.23728 px Iteration 2: RMSD = 0.633676 px Iteration 3: RMSD = 0.163426 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 2.05012 px Iteration 2: RMSD = 0.659717 px Iteration 3: RMSD = 0.157852 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 3.53341 px Iteration 2: RMSD = 0.639566 px Iteration 3: RMSD = 0.206295 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 31.15 px Iteration 2: RMSD = 0.632749 px Iteration 3: RMSD = 0.244067 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 3.16889 px Iteration 2: RMSD = 0.685643 px Iteration 3: RMSD = 0.218279 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 2.41167 px Iteration 2: RMSD = 0.643544 px Iteration 3: RMSD = 0.140381 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 2.14527 px Iteration 2: RMSD = 0.359359 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 2.46885 px Iteration 2: RMSD = 0.762707 px Iteration 3: RMSD = 0.220733 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 3.66996 px Iteration 2: RMSD = 0.653044 px Iteration 3: RMSD = 0.459396 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 3.14333 px Iteration 2: RMSD = 0.522321 px Iteration 3: RMSD = 0.39446 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 2.43824 px Iteration 2: RMSD = 0.453186 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 1.96107 px Iteration 2: RMSD = 0.353814 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 2.15575 px Iteration 2: RMSD = 0.534675 px Iteration 3: RMSD = 0.181721 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 1.93205 px Iteration 2: RMSD = 0.537494 px Iteration 3: RMSD = 0.209229 px Polynomial fit RMSD: X = 8.71114 px Y = 2.32456 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/278-1_003.mrc