Working on 273_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 25.5404 Std = 5.63384 Hotpixel threshold = 59.3434 Detected 256 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 3.80523 px Iteration 2: RMSD = 0.763553 px Iteration 3: RMSD = 0.220816 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 5.40239 px Iteration 2: RMSD = 0.864367 px Iteration 3: RMSD = 0.412139 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 5.13544 px Iteration 2: RMSD = 1.76659 px Iteration 3: RMSD = 0.685019 px Iteration 4: RMSD = 0.344444 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 5.11837 px Iteration 2: RMSD = 3.16231 px Iteration 3: RMSD = 4.40169 px Iteration 4: RMSD = 2.78775 px Iteration 5: RMSD = 2.86513 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 11.5284 px Iteration 2: RMSD = 7.26494 px Iteration 3: RMSD = 5.12637 px Iteration 4: RMSD = 4.69473 px Iteration 5: RMSD = 0.488376 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 12.7117 px Iteration 2: RMSD = 11.7948 px Iteration 3: RMSD = 8.12805 px Iteration 4: RMSD = 8.23217 px Iteration 5: RMSD = 8.69523 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 4.02394 px Iteration 2: RMSD = 3.8027 px Iteration 3: RMSD = 1.41717 px Iteration 4: RMSD = 1.2424 px Iteration 5: RMSD = 1.19928 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 5.25556 px Iteration 2: RMSD = 0.723472 px Iteration 3: RMSD = 0.41625 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 4.65742 px Iteration 2: RMSD = 3.70834 px Iteration 3: RMSD = 3.6637 px Iteration 4: RMSD = 0.755785 px Iteration 5: RMSD = 0.353311 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 10.1147 px Iteration 2: RMSD = 10.1863 px Iteration 3: RMSD = 9.46539 px Iteration 4: RMSD = 9.51639 px Iteration 5: RMSD = 9.47301 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 10.8227 px Iteration 2: RMSD = 10.986 px Iteration 3: RMSD = 8.40521 px Iteration 4: RMSD = 7.61576 px Iteration 5: RMSD = 8.11249 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 3.14186 px Iteration 2: RMSD = 0.847055 px Iteration 3: RMSD = 0.321164 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 2.49598 px Iteration 2: RMSD = 1.07009 px Iteration 3: RMSD = 0.343097 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 2.70878 px Iteration 2: RMSD = 1.08178 px Iteration 3: RMSD = 0.422824 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 8.10289 px Iteration 2: RMSD = 2.37306 px Iteration 3: RMSD = 0.755785 px Iteration 4: RMSD = 0.395454 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 12.5155 px Iteration 2: RMSD = 7.33601 px Iteration 3: RMSD = 9.71437 px Iteration 4: RMSD = 9.62417 px Iteration 5: RMSD = 10.1019 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 3.57537 px Iteration 2: RMSD = 0.845584 px Iteration 3: RMSD = 0.292174 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 2.44198 px Iteration 2: RMSD = 0.566095 px Iteration 3: RMSD = 0.253916 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 7.10707 px Iteration 2: RMSD = 2.22027 px Iteration 3: RMSD = 0.762406 px Iteration 4: RMSD = 0.352749 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 5.51684 px Iteration 2: RMSD = 1.3029 px Iteration 3: RMSD = 0.61761 px Iteration 4: RMSD = 0.288333 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 8.26724 px Iteration 2: RMSD = 6.77036 px Iteration 3: RMSD = 6.64071 px Iteration 4: RMSD = 6.51529 px Iteration 5: RMSD = 6.43166 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 3.95121 px Iteration 2: RMSD = 0.603828 px Iteration 3: RMSD = 0.155311 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 2.93963 px Iteration 2: RMSD = 0.715249 px Iteration 3: RMSD = 0.206409 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 2.15366 px Iteration 2: RMSD = 0.701287 px Iteration 3: RMSD = 0.177918 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 5.19712 px Iteration 2: RMSD = 1.20062 px Iteration 3: RMSD = 0.369747 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 3.58609 px Iteration 2: RMSD = 2.19082 px Iteration 3: RMSD = 3.7269 px Iteration 4: RMSD = 1.345 px Iteration 5: RMSD = 1.10218 px Polynomial fit RMSD: X = 4.27112 px Y = 3.64486 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/273_003.mrc