Working on 273_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.4858 Std = 5.62242 Hotpixel threshold = 60.2203 Detected 303 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 4.78944 px Iteration 2: RMSD = 0.464685 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 4.79043 px Iteration 2: RMSD = 2.56315 px Iteration 3: RMSD = 0.906312 px Iteration 4: RMSD = 0.609023 px Iteration 5: RMSD = 0.293311 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 5.53193 px Iteration 2: RMSD = 1.83751 px Iteration 3: RMSD = 0.538894 px Iteration 4: RMSD = 0.380268 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 3.56284 px Iteration 2: RMSD = 2.91028 px Iteration 3: RMSD = 0.619349 px Iteration 4: RMSD = 0.259508 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 3.41102 px Iteration 2: RMSD = 0.619348 px Iteration 3: RMSD = 0.253079 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 3.01746 px Iteration 2: RMSD = 0.52451 px Iteration 3: RMSD = 0.178693 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 3.23594 px Iteration 2: RMSD = 1.32318 px Iteration 3: RMSD = 0.462344 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 4.47433 px Iteration 2: RMSD = 1.91023 px Iteration 3: RMSD = 0.680926 px Iteration 4: RMSD = 0.407235 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 11.3712 px Iteration 2: RMSD = 5.8722 px Iteration 3: RMSD = 1.39589 px Iteration 4: RMSD = 0.404876 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 8.9362 px Iteration 2: RMSD = 8.71326 px Iteration 3: RMSD = 8.78278 px Iteration 4: RMSD = 7.13796 px Iteration 5: RMSD = 6.08298 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 3.36868 px Iteration 2: RMSD = 1.39856 px Iteration 3: RMSD = 0.417751 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 21.8466 px Iteration 2: RMSD = 22.2418 px Iteration 3: RMSD = 21.064 px Iteration 4: RMSD = 19.4895 px Iteration 5: RMSD = 20.1444 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 12.5025 px Iteration 2: RMSD = 11.5946 px Iteration 3: RMSD = 6.02249 px Iteration 4: RMSD = 5.43042 px Iteration 5: RMSD = 5.36832 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 3.18438 px Iteration 2: RMSD = 1.12764 px Iteration 3: RMSD = 1.26663 px Iteration 4: RMSD = 0.541207 px Iteration 5: RMSD = 0.349608 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 4.51392 px Iteration 2: RMSD = 1.09467 px Iteration 3: RMSD = 0.561228 px Iteration 4: RMSD = 0.218725 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 4.19813 px Iteration 2: RMSD = 1.48383 px Iteration 3: RMSD = 1.30515 px Iteration 4: RMSD = 1.18242 px Iteration 5: RMSD = 1.22131 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 13.318 px Iteration 2: RMSD = 7.39295 px Iteration 3: RMSD = 3.88738 px Iteration 4: RMSD = 3.78442 px Iteration 5: RMSD = 3.52958 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 9.66745 px Iteration 2: RMSD = 5.69689 px Iteration 3: RMSD = 4.75327 px Iteration 4: RMSD = 2.89544 px Iteration 5: RMSD = 0.964565 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 4.33933 px Iteration 2: RMSD = 4.68932 px Iteration 3: RMSD = 4.4833 px Iteration 4: RMSD = 4.50205 px Iteration 5: RMSD = 4.48442 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 4.76473 px Iteration 2: RMSD = 0.751475 px Iteration 3: RMSD = 0.214596 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 2.79399 px Iteration 2: RMSD = 0.831028 px Iteration 3: RMSD = 0.373201 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 7.47144 px Iteration 2: RMSD = 6.45082 px Iteration 3: RMSD = 0.610339 px Iteration 4: RMSD = 0.214532 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 3.1595 px Iteration 2: RMSD = 0.968403 px Iteration 3: RMSD = 0.590308 px Iteration 4: RMSD = 0.252333 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.55693 px Iteration 2: RMSD = 1.74729 px Iteration 3: RMSD = 0.760431 px Iteration 4: RMSD = 0.329028 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 2.4893 px Iteration 2: RMSD = 0.679711 px Iteration 3: RMSD = 0.207179 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 3.73418 px Iteration 2: RMSD = 0.512351 px Iteration 3: RMSD = 0.207624 px Polynomial fit RMSD: X = 3.41265 px Y = 3.49844 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/273_002.mrc