Working on 272_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 25.894 Std = 5.5109 Hotpixel threshold = 58.9594 Detected 285 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 5.50627 px Iteration 2: RMSD = 1.14412 px Iteration 3: RMSD = 0.357209 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 32.9343 px Iteration 2: RMSD = 19.5454 px Iteration 3: RMSD = 19.8065 px Iteration 4: RMSD = 16.1421 px Iteration 5: RMSD = 17.4285 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 10.3365 px Iteration 2: RMSD = 11.4921 px Iteration 3: RMSD = 10.749 px Iteration 4: RMSD = 11.1026 px Iteration 5: RMSD = 9.83156 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 15.8827 px Iteration 2: RMSD = 3.32902 px Iteration 3: RMSD = 2.36346 px Iteration 4: RMSD = 2.3452 px Iteration 5: RMSD = 2.324 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 12.0878 px Iteration 2: RMSD = 1.28733 px Iteration 3: RMSD = 1.20611 px Iteration 4: RMSD = 0.955658 px Iteration 5: RMSD = 0.939433 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 21.7729 px Iteration 2: RMSD = 3.96624 px Iteration 3: RMSD = 0.644226 px Iteration 4: RMSD = 0.216653 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 68.6675 px Iteration 2: RMSD = 68.2757 px Iteration 3: RMSD = 64.6511 px Iteration 4: RMSD = 18.9019 px Iteration 5: RMSD = 19.1616 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 27.729 px Iteration 2: RMSD = 16.7339 px Iteration 3: RMSD = 17.613 px Iteration 4: RMSD = 15.7 px Iteration 5: RMSD = 14.8239 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 18.316 px Iteration 2: RMSD = 2.09111 px Iteration 3: RMSD = 0.62568 px Iteration 4: RMSD = 0.269147 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 10.868 px Iteration 2: RMSD = 5.2443 px Iteration 3: RMSD = 0.952024 px Iteration 4: RMSD = 0.386932 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 5.37553 px Iteration 2: RMSD = 4.16494 px Iteration 3: RMSD = 0.922875 px Iteration 4: RMSD = 0.205619 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 20.535 px Iteration 2: RMSD = 13.0074 px Iteration 3: RMSD = 13.1984 px Iteration 4: RMSD = 6.42188 px Iteration 5: RMSD = 6.39862 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 16.6954 px Iteration 2: RMSD = 8.25644 px Iteration 3: RMSD = 3.17952 px Iteration 4: RMSD = 0.42899 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 15.9231 px Iteration 2: RMSD = 10.7379 px Iteration 3: RMSD = 11.4621 px Iteration 4: RMSD = 10.426 px Iteration 5: RMSD = 11.1919 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 14.8708 px Iteration 2: RMSD = 5.05966 px Iteration 3: RMSD = 1.4913 px Iteration 4: RMSD = 0.412937 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 7.78666 px Iteration 2: RMSD = 1.48997 px Iteration 3: RMSD = 0.741522 px Iteration 4: RMSD = 0.207874 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 42.3635 px Iteration 2: RMSD = 13.49 px Iteration 3: RMSD = 12.4957 px Iteration 4: RMSD = 12.6967 px Iteration 5: RMSD = 10.9383 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 16.5957 px Iteration 2: RMSD = 35.7489 px Iteration 3: RMSD = 11.7159 px Iteration 4: RMSD = 10.7133 px Iteration 5: RMSD = 10.6493 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 21.0265 px Iteration 2: RMSD = 1.13866 px Iteration 3: RMSD = 0.444555 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 3.65221 px Iteration 2: RMSD = 2.24227 px Iteration 3: RMSD = 0.528814 px Iteration 4: RMSD = 0.196086 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 26.1524 px Iteration 2: RMSD = 4.68933 px Iteration 3: RMSD = 0.952018 px Iteration 4: RMSD = 0.282406 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 25.6307 px Iteration 2: RMSD = 13.3162 px Iteration 3: RMSD = 14.1882 px Iteration 4: RMSD = 14.8714 px Iteration 5: RMSD = 14.8752 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 20.9798 px Iteration 2: RMSD = 4.91841 px Iteration 3: RMSD = 1.25211 px Iteration 4: RMSD = 0.638297 px Iteration 5: RMSD = 0.391765 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 40.5443 px Iteration 2: RMSD = 7.90099 px Iteration 3: RMSD = 0.840458 px Iteration 4: RMSD = 0.307317 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 13.5949 px Iteration 2: RMSD = 5.24348 px Iteration 3: RMSD = 4.97186 px Iteration 4: RMSD = 4.9795 px Iteration 5: RMSD = 4.94082 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 5.7985 px Iteration 2: RMSD = 0.939789 px Iteration 3: RMSD = 0.467127 px Polynomial fit RMSD: X = 15.6439 px Y = 13.274 px The polynomial motion model did not explain the observation very well. Local correction is disabled for this micrograph. Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/272_002.mrc