Working on 271_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.3459 Std = 5.60703 Hotpixel threshold = 59.9881 Detected 303 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 9.87946 px Iteration 2: RMSD = 0.795497 px Iteration 3: RMSD = 0.200248 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 28.9056 px Iteration 2: RMSD = 15.5835 px Iteration 3: RMSD = 14.8925 px Iteration 4: RMSD = 13.2026 px Iteration 5: RMSD = 12.3966 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 14.3059 px Iteration 2: RMSD = 12.6582 px Iteration 3: RMSD = 11.1938 px Iteration 4: RMSD = 10.9706 px Iteration 5: RMSD = 9.82868 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 6.34464 px Iteration 2: RMSD = 5.03224 px Iteration 3: RMSD = 5.18363 px Iteration 4: RMSD = 5.1953 px Iteration 5: RMSD = 5.24754 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 8.78565 px Iteration 2: RMSD = 6.07795 px Iteration 3: RMSD = 8.05808 px Iteration 4: RMSD = 7.97917 px Iteration 5: RMSD = 7.93106 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 7.01344 px Iteration 2: RMSD = 6.06305 px Iteration 3: RMSD = 1.11274 px Iteration 4: RMSD = 0.358268 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 38.1904 px Iteration 2: RMSD = 33.4318 px Iteration 3: RMSD = 34.5706 px Iteration 4: RMSD = 33.3342 px Iteration 5: RMSD = 33.0167 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 17.9514 px Iteration 2: RMSD = 15.8241 px Iteration 3: RMSD = 14.0257 px Iteration 4: RMSD = 11.1649 px Iteration 5: RMSD = 11.3393 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 15.0593 px Iteration 2: RMSD = 13.3341 px Iteration 3: RMSD = 10.8098 px Iteration 4: RMSD = 8.24697 px Iteration 5: RMSD = 5.63411 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.6315 px Iteration 2: RMSD = 7.28622 px Iteration 3: RMSD = 5.07281 px Iteration 4: RMSD = 1.2302 px Iteration 5: RMSD = 1.18749 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 17.3687 px Iteration 2: RMSD = 6.86186 px Iteration 3: RMSD = 6.95997 px Iteration 4: RMSD = 4.37682 px Iteration 5: RMSD = 3.38652 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 16.2411 px Iteration 2: RMSD = 15.8672 px Iteration 3: RMSD = 13.7538 px Iteration 4: RMSD = 11.4262 px Iteration 5: RMSD = 11.6185 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 13.4839 px Iteration 2: RMSD = 6.80179 px Iteration 3: RMSD = 5.62746 px Iteration 4: RMSD = 4.02768 px Iteration 5: RMSD = 4.11523 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 15.6616 px Iteration 2: RMSD = 8.24512 px Iteration 3: RMSD = 7.69143 px Iteration 4: RMSD = 7.45423 px Iteration 5: RMSD = 7.44397 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 11.8836 px Iteration 2: RMSD = 9.95999 px Iteration 3: RMSD = 8.63693 px Iteration 4: RMSD = 7.7615 px Iteration 5: RMSD = 7.82099 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 23.1277 px Iteration 2: RMSD = 1.05019 px Iteration 3: RMSD = 0.57504 px Iteration 4: RMSD = 0.251425 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 15.9704 px Iteration 2: RMSD = 7.23386 px Iteration 3: RMSD = 6.18849 px Iteration 4: RMSD = 4.90376 px Iteration 5: RMSD = 4.96144 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 17.2264 px Iteration 2: RMSD = 7.87182 px Iteration 3: RMSD = 5.59253 px Iteration 4: RMSD = 5.13355 px Iteration 5: RMSD = 4.03353 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 15.6004 px Iteration 2: RMSD = 11.5899 px Iteration 3: RMSD = 10.1817 px Iteration 4: RMSD = 10.2902 px Iteration 5: RMSD = 8.15614 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 24.8374 px Iteration 2: RMSD = 6.17075 px Iteration 3: RMSD = 1.1708 px Iteration 4: RMSD = 0.80183 px Iteration 5: RMSD = 0.476869 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 7.05375 px Iteration 2: RMSD = 0.789107 px Iteration 3: RMSD = 0.46294 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 14.5021 px Iteration 2: RMSD = 7.16738 px Iteration 3: RMSD = 4.58508 px Iteration 4: RMSD = 4.06647 px Iteration 5: RMSD = 3.94555 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 14.37 px Iteration 2: RMSD = 7.73243 px Iteration 3: RMSD = 10.831 px Iteration 4: RMSD = 4.40428 px Iteration 5: RMSD = 2.99998 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 13.2009 px Iteration 2: RMSD = 7.54896 px Iteration 3: RMSD = 6.55545 px Iteration 4: RMSD = 3.21243 px Iteration 5: RMSD = 3.19273 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 10.821 px Iteration 2: RMSD = 6.45284 px Iteration 3: RMSD = 6.06293 px Iteration 4: RMSD = 1.04944 px Iteration 5: RMSD = 0.481385 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 36.7446 px Iteration 2: RMSD = 1.11428 px Iteration 3: RMSD = 0.322531 px Polynomial fit RMSD: X = 6.50248 px Y = 18.7386 px The polynomial motion model did not explain the observation very well. Local correction is disabled for this micrograph. Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/271_002.mrc