Working on 268_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.3132 Std = 5.58147 Hotpixel threshold = 59.802 Detected 294 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 14.2329 px Iteration 2: RMSD = 1.84123 px Iteration 3: RMSD = 0.141468 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 29.5348 px Iteration 2: RMSD = 9.98341 px Iteration 3: RMSD = 7.12841 px Iteration 4: RMSD = 5.14734 px Iteration 5: RMSD = 4.43324 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 10.7557 px Iteration 2: RMSD = 5.46413 px Iteration 3: RMSD = 2.74119 px Iteration 4: RMSD = 1.83081 px Iteration 5: RMSD = 1.7835 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 8.87235 px Iteration 2: RMSD = 2.02867 px Iteration 3: RMSD = 1.72076 px Iteration 4: RMSD = 1.14069 px Iteration 5: RMSD = 1.09418 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 8.23419 px Iteration 2: RMSD = 2.07337 px Iteration 3: RMSD = 0.888834 px Iteration 4: RMSD = 0.4357 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 32.3778 px Iteration 2: RMSD = 1.11121 px Iteration 3: RMSD = 0.301188 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 19.2411 px Iteration 2: RMSD = 6.41819 px Iteration 3: RMSD = 6.36957 px Iteration 4: RMSD = 6.23751 px Iteration 5: RMSD = 6.37657 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 3.78561 px Iteration 2: RMSD = 2.42194 px Iteration 3: RMSD = 0.677538 px Iteration 4: RMSD = 0.620909 px Iteration 5: RMSD = 0.172428 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 12.0401 px Iteration 2: RMSD = 2.1163 px Iteration 3: RMSD = 0.549606 px Iteration 4: RMSD = 0.315003 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 15.7739 px Iteration 2: RMSD = 5.46623 px Iteration 3: RMSD = 2.34403 px Iteration 4: RMSD = 2.06234 px Iteration 5: RMSD = 2.23993 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 26.3425 px Iteration 2: RMSD = 1.59392 px Iteration 3: RMSD = 0.588144 px Iteration 4: RMSD = 0.299209 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 17.2711 px Iteration 2: RMSD = 13.5837 px Iteration 3: RMSD = 13.924 px Iteration 4: RMSD = 12.0099 px Iteration 5: RMSD = 11.1027 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 11.7114 px Iteration 2: RMSD = 1.80746 px Iteration 3: RMSD = 2.55749 px Iteration 4: RMSD = 4.93446 px Iteration 5: RMSD = 0.826971 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 12.2379 px Iteration 2: RMSD = 1.40705 px Iteration 3: RMSD = 0.616204 px Iteration 4: RMSD = 0.306744 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 10.1916 px Iteration 2: RMSD = 3.28925 px Iteration 3: RMSD = 1.08936 px Iteration 4: RMSD = 0.445023 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 55.2328 px Iteration 2: RMSD = 1.09406 px Iteration 3: RMSD = 0.179268 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 6.80662 px Iteration 2: RMSD = 1.43157 px Iteration 3: RMSD = 1.14703 px Iteration 4: RMSD = 0.684904 px Iteration 5: RMSD = 0.608767 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 10.1713 px Iteration 2: RMSD = 4.33633 px Iteration 3: RMSD = 1.62677 px Iteration 4: RMSD = 5.35758 px Iteration 5: RMSD = 5.36649 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 12.1964 px Iteration 2: RMSD = 1.11469 px Iteration 3: RMSD = 0.58076 px Iteration 4: RMSD = 0.188239 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 9.10786 px Iteration 2: RMSD = 1.84377 px Iteration 3: RMSD = 0.985629 px Iteration 4: RMSD = 0.800633 px Iteration 5: RMSD = 0.856468 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 32.3057 px Iteration 2: RMSD = 32.4054 px Iteration 3: RMSD = 0.753593 px Iteration 4: RMSD = 0.167529 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 13.4245 px Iteration 2: RMSD = 5.83735 px Iteration 3: RMSD = 2.82481 px Iteration 4: RMSD = 2.64622 px Iteration 5: RMSD = 0.941755 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 14.3731 px Iteration 2: RMSD = 4.83211 px Iteration 3: RMSD = 3.98589 px Iteration 4: RMSD = 1.0448 px Iteration 5: RMSD = 0.305232 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 10.5153 px Iteration 2: RMSD = 0.933722 px Iteration 3: RMSD = 0.381213 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 8.34667 px Iteration 2: RMSD = 1.09542 px Iteration 3: RMSD = 0.37445 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 1.95425 px Iteration 2: RMSD = 1.0027 px Iteration 3: RMSD = 0.242732 px Polynomial fit RMSD: X = 8.06232 px Y = 19.5688 px The polynomial motion model did not explain the observation very well. Local correction is disabled for this micrograph. Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/268_002.mrc